Evaluation of Hepatic Steatosis in Rodents by Time-Domain Nuclear Magnetic Resonance

Autor: Jose Donato, José Cipolla-Neto, Rafaela S. Pinto, João A.B. Pedroso, Joao Paulo Camporez, Luciana Tocci Belpiede
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Diagnostics
Volume 9
Issue 4
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
Diagnostics, Vol 9, Iss 4, p 198 (2019)
ISSN: 2075-4418
DOI: 10.3390/diagnostics9040198
Popis: Devices that analyze body composition of rodents by time-domain nuclear magnetic resonance (TD-NMR) are becoming popular in research centers that study metabolism. Theoretically, TD-NMR devices can also evaluate lipid content in isolated tissues. However, the accuracy of TD-NMR to determine hepatic steatosis in the liver of small laboratory animals has not been evaluated in detail. We observed that TD-NMR was able to detect increased lipid content in the liver of rats consuming high-fat diet (HFD) for 12 weeks and in genetically obese (Lepob/ob and Leprdb/db) mice. The lipid content determined by TD-NMR showed a positive correlation with triglyceride content measured by colorimetric assays. In contrast, TD-NMR did not detect hepatic steatosis in C57BL/6 mice consuming HFD for 4 or 12 weeks, despite their obesity and increased liver triglyceride content. These findings indicate that tissue mass and the severity of hepatic steatosis affect the sensitivity of TD-NMR to detect liver lipid content.
Databáze: OpenAIRE
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