GDM Alters Expression of Placental Estrogen Receptor α in a Cell Type and Gender-Specific Manner
| Autor: | Christina Riedel, R Hüttenbrenner, Stefan Hutter, Verena Kirn, Udo Jeschke, Julia Knabl, Ursula Hiden, Margit Günthner-Biller, Franz Kainer, Gernot Desoye |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
Male medicine.medical_specialty medicine.drug_class Placenta Down-Regulation Estrogen receptor Gestational Age Biology Real-Time Polymerase Chain Reaction Epigenesis Genetic Fetus Sex Factors Syncytiotrophoblast Downregulation and upregulation Pregnancy Internal medicine medicine Humans RNA Messenger Promoter Regions Genetic Estrogen receptor beta Messenger RNA Reverse Transcriptase Polymerase Chain Reaction Estrogen Receptor alpha Gene Expression Regulation Developmental Obstetrics and Gynecology Trophoblast DNA Methylation Sex Determination Processes Immunohistochemistry Trophoblasts Diabetes Gestational medicine.anatomical_structure Endocrinology Nuclear receptor Estrogen Case-Control Studies Blood Vessels CpG Islands Female |
| Zdroj: | Reproductive Sciences. 22:1488-1495 |
| ISSN: | 1933-7205 1933-7191 |
| Popis: | The nuclear receptor estrogen receptor α (ERα) is one of the key players in energy balance, insulin resistance, and trophoblast differentiation. We tested the hypothesis that gestational diabetes mellitus (GDM) alters expression of placental ERα in a cell type-specific manner and that this regulation may involve epigenetic changes.Expression of ERα was analyzed by immunohistochemistry using the semiquantitative immunoreactive score in 80 placentas (40 GDM/40 controls). Quantitative real-time polymerase chain reaction (PCR) measured ERα messenger RNA (mRNA) in decidual tissue. Methylation-specific PCR was performed to analyze cytosine-phosphatidyl-guanine-island methylation of the ERα promoter.Expression of ERα protein is upregulated (P = .011) in GDM in extravillous trophoblasts but not in syncytiotrophoblast. Gestational diabetes mellitus downregulated ERα in decidual vessels only in pregnancies with male but not female fetuses. Furthermore, mRNA of the ERα encoding gene estrogen receptor gene 1 (ESR1) was increased (+1.77 fold) in GDM decidua when compared to controls (P = .024). In parallel, the promoter of ESR1 was methylated only in decidua of healthy control individuals but not in GDM.Gestational diabetes mellitus affects expression of placental ERα in a cell type-dependent way, on epigenetic level. These data link GDM with epigenetic deregulations of ERα expression and open new insights into the intrauterine programming hypothesis of GDM. |
| Databáze: | OpenAIRE |
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