Intraoperative probe-directed immunodetection using a monoclonal antibody
| Autor: | William B. Farrar, George H. Hinkle, Marlin O. Thurston, Michel Rousseau, Patrick J. O'Dwyer, Edward W. Martin, John O. Olsen, Rolf F. Barth, Cathy Mojzisik, Daniel P. McCabe, Steven E. Tuttle |
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| Rok vydání: | 1986 |
| Předmět: |
Adult
Male medicine.medical_specialty Colorectal cancer medicine.drug_class Rectum Mice Nude Monoclonal antibody Palpation Iodine Radioisotopes Immunoglobulin Fab Fragments Mice medicine Animals Humans Large intestine Radioimmunoguided surgery Subclinical infection Intraoperative Care medicine.diagnostic_test biology business.industry Rectal Neoplasms Antibodies Monoclonal Middle Aged medicine.disease Surgery medicine.anatomical_structure Evaluation Studies as Topic Colonic Neoplasms biology.protein Scintillation Counting Female Antibody business |
| Zdroj: | Archives of surgery (Chicago, Ill. : 1960). 121(12) |
| ISSN: | 0004-0010 |
| Popis: | To assess monoclonal antibody (MAb) 17-1A and its F(ab')2 fragment in intraoperative radioimmunodetection and to evaluate further the clinical usefulness of a hand-held gamma-detecting probe (GDP), we injected radiolabeled monoclonal antibody 17-1A three to six days preoperatively or its F(ab')2 fragment two to three days preoperatively into 18 patients with colorectal cancer. Intraoperative GDP counts with tumor-tissue ratios of 1.5:1 or greater were obtained from 15 (75%) of 20 tumor sites, with ratios averaging 2.3:1 for fragments and 3.4:1 for whole antibody. The GDP counts contributed to intraoperative decision making in three patients, either by localization of tumor not identified by inspection or palpation or by mapping margins of resection with histologic confirmation of a local/regional recurrence. These preliminary data demonstrate that probe-directed, intraoperative radioimmunodetection can assist the surgeon in detecting subclinical tumor deposits and thus better evaluate the extent of primary or recurrent colorectal cancers intraoperatively. |
| Databáze: | OpenAIRE |
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