Evidence for the cholinergic markers ChAT and vAChT in sensory cells of the developing antennal nervous system of the desert locust Schistocerca gregaria
| Autor: | Erica Ehrhardt, George Boyan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Nervous system animal structures Campaniform sensilla Vesicular Acetylcholine Transport Proteins Grasshoppers Development Nervous System Choline O-Acetyltransferase vAChT 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Developmental Neuroscience Vesicular acetylcholine transporter medicine Animals Sensilla biology fungi biology.organism_classification Choline acetyltransferase Locust Cell biology 030104 developmental biology medicine.anatomical_structure nervous system Sensory cells ChAT Antenna Cholinergic Nerve tract Schistocerca Original Article sense organs 030217 neurology & neurosurgery |
| Zdroj: | Invertebrate Neuroscience |
| ISSN: | 1439-1104 1354-2516 |
| Popis: | Sensory and motor systems in insects with hemimetabolous development must be ready to mediate adaptive behavior directly on hatching from the egg. For the desert locust S. gregaria, cholinergic transmission from antennal sensillae to olfactory or mechanosensory centers in the brain requires that choline acetyltransferase (ChAT) and the vesicular acetylcholine transporter (vAChT) already be present in sensory cells in the first instar. In this study, we used immunolabeling to demonstrate that ChAT and vAChT are both expressed in sensory cells from identifiable sensilla types in the immature antennal nervous system. We observed ChAT expression in dendrites, neurites and somata of putative basiconic-type sensillae at the first instar stage. We also detected vAChT in the sensory axons of these sensillae in a major antennal nerve tract. We then examined whether evidence for cholinergic transmission is present during embryogenesis. Immunolabeling confirms that vAChT is expressed in somata typical of campaniform sensillae, as well as in small sensory cell clusters typically associated with either a large basiconic or coeloconic sensilla, at 99% of embryogenesis. The vAChT is also expressed in the somata of these sensilla types in multiple antennal regions at 90% of embryogenesis, but not at earlier (70%) embryonic stages. Neuromodulators are known to appear late in embryogenesis in neurons of the locust central complex, and the cholinergic system of the antenna may also only reach maturity shortly before hatching. |
| Databáze: | OpenAIRE |
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