Liquid Chromatographic Methods for Vitamins A and D in Multivitamin-Mineral Formulations
| Autor: | Normand Beaulieu, Norman M. Curran, Micheline Gravelle, Charles Gagné, Edward G Lovering |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Vitamin
Minerals Chromatography General Chemistry Retinyl acetate Hexane chemistry.chemical_compound Ergocalciferol chemistry Retinyl palmitate Vitamin D and neurology medicine Spectrophotometry Ultraviolet Vitamin D Vitamin A Cholecalciferol Chromatography High Pressure Liquid Screening procedures medicine.drug |
| Zdroj: | Journal of AOAC INTERNATIONAL. 72:247-254 |
| ISSN: | 0004-5756 |
| DOI: | 10.1093/jaoac/72.2.247 |
| Popis: | Liquid chromatographic screening procedures have been developed for the estimation of vitamins A and D in multivitamin-mineral tablet, capsule, gelatin capsule, and syrup formulations. The procedure can be used for measuring vitamin A present as either retinyl acetate or retinyl palmitate, and also for measuring the contribution to total vitamin A activity from 13-cis retinyl esters. The retinyl esters and their isomers are resolved from each other and their oxidation products. Ergocalciferol and cholecalciferol are not resolved from each other but they are resolved from other vitamin D isomers and from vitamins A, E, and K and their degradation products. Both assays use a 3 μm amino column, with a mobile phase of hexane for vitamin A and 1% isopropanol in hexane for vitamin D. The precision of replicate injections for vitamins A and D is better than 1% and the recovery from spiked syrups is better than 98%. The coefficient of variation for both assay methods is about 5%. Twenty formulations were analyzed for vitamin A and 24 were analyzed for vitamin D. |
| Databáze: | OpenAIRE |
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