Longitudinal Genomic Characterization of Carbapenemase-producing Enterobacteriaceae (CPE) Reveals Changing Pattern of CPE Isolated in Hong Kong Hospitals
| Autor: | Kitty S. C. Fung, Carmen Li, Christopher K C Lai, Nilakshi Barua, Mamie Hui, Veranja Liyanapathirana, Ying Yang, Dominic N.C. Tsang, Rita W Y Ng, Norman Lo, Raymond Lai, Chendi Zhu, Kam Tak Wong, Margaret Ip, Ka Yee Chau |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Carbapenemase-Producing Enterobacteriaceae Klebsiella pneumoniae Microbial Sensitivity Tests Yersiniabactin beta-Lactamases Microbiology chemistry.chemical_compound Plasmid Bacterial Proteins Drug Resistance Multiple Bacterial Escherichia coli Humans Pharmacology (medical) Whole genome sequencing biology Molecular epidemiology Whole Genome Sequencing Incidence (epidemiology) Enterobacteriaceae Infections General Medicine biology.organism_classification Enterobacteriaceae Anti-Bacterial Agents Interspersed Repetitive Sequences Infectious Diseases Carbapenem-Resistant Enterobacteriaceae chemistry Hong Kong Genome Bacterial Multilocus Sequence Typing Plasmids |
| Zdroj: | International journal of antimicrobial agents. 58(5) |
| ISSN: | 1872-7913 |
| Popis: | A rising trend of carbapenemase-producing Enterobacteriaceae (CPE) isolated from patients was observed after implementing an active CPE surveillance programme in Hong Kong hospitals from 2011. We described the molecular epidemiology of 567 CPE from patients of three of seven public hospital clusters in Hong Kong. During a 7-year period, the incidence of CPE isolation increased from 0.05 to 9.6 /100,000 patient-days. The carbapenemase genes identified were polyclonal, including blaKPC, blaNDM and blaIMP which were mainly associated with hospitalization overseas in earlier years. However, increasing CPE isolation from patients without overseas hospitalization occurred in 2015, with blaNDM (55.1%) becoming predominant followed by and blaIMP (30.0%). E. coli (46.4%) and Klebsiella spp. (38.3%) were the dominant species. Whole genome sequencing was performed on 169 representative isolates with a combination of short and long reads using Illumina and Nanopore technology. Two distinct lineages of blaKPC-2-positive Klebsiella pneumoniae (ST11&ST258) were identified with ST11 carrying yersiniabactin gene ybt-9 on ICEKp3. ST131 E. coli producing IMP-4 was present throughout the study period. The blaNDM and blaIMP genes were mainly carried in IncX3 and IncN-ST7 plasmids respectively. blaOXA-48-like gene was carried in IncX3 plasmid in E. coli but ColKP3 plasmid in K. pneumoniae. A lineage of K. pneumoniae with blaNDM-1 plus blaOXA-232 in distinct plasmids of IncF1B/IncHI1B was identified and associated with prior overseas hospitalization. This study highlights the threat of multiple types of CPE present, with the predominance of blaNDM and blaIMP among CPE in our hospitals. Enhanced containment strategies are needed to mitigate the rapidly rising trends of CPE in healthcare settings. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect