Salt-induced expression of NADP-dependent isocitrate dehydrogenase and ferredoxin-dependent glutamate synthase in Mesembryanthemum crystallinum
| Autor: | Karl-Josef Dietz, Dortje Golldack, T. N. Popova, Stanislav F. Ismailov, Olga V. Popova |
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| Rok vydání: | 2002 |
| Předmět: |
Nitrogen
Molecular Sequence Data Plant Science Sodium Chloride Biology Gene Expression Regulation Enzymologic chemistry.chemical_compound tissue-specific Biosynthesis Gene Expression Regulation Plant Halophyte Glutamate synthase expression Genetics Amino Acid Sequence salt stress chemistry.chemical_classification Mesembryanthemum Sequence Homology Amino Acid ferredoxin-dependent glutamate synthase Mesembryanthemum crystallinum Metabolism biology.organism_classification Adaptation Physiological Immunohistochemistry Carbon Isocitrate Dehydrogenase Enzyme Isocitrate dehydrogenase chemistry Biochemistry Aizoaceae biology.protein Amino Acid Oxidoreductases proline accumulation |
| Zdroj: | Planta. 215:906-913 |
| ISSN: | 1432-2048 0032-0935 |
| DOI: | 10.1007/s00425-002-0827-0 |
| Popis: | NADP-specific isocitrate dehydrogenase is a key cytosolic enzyme that links C and N metabolism by supplying C skeletons for primary N assimilation in plants. We report the characterization of the transcript Mc-ICDH1 encoding an NADP-dependent isocitrate dehydrogenase (NADP-ICDH, EC 1.1.1.42) from the facultative halophyte Mesembryanthemum crystallinum L., focussing on salt-dependent regulation of the enzyme. The activity of NADP-ICDH in plants adapted to high salinity increased in leaves and decreased in roots. By transcript analyses and Western-type hybridizations, expression of Mc-ICDH1 was found to be stimulated in leaves in salt-adapted M. crystallinum. By immunocytological analyses, NADP-ICDH proteins were localized to most cell types with strongest expression in epidermal cells and in the vascular tissue. In leaves of salt-adapted plants, signal intensities increased in mesophyll cells. In contrast to Mc-ICDH1, the activity and transcript abundance of ferredoxin-dependent glutamate synthase (Fd-GOGAT, EC 1.4.7.1), which is the key enzyme of N assimilation and biosynthesis of amino acids, decreased in leaves in response to salt stress. The physiological roles of NADP-ICDH and Fd-GOGAT in the adaptation of plants to high salinity are discussed. |
| Databáze: | OpenAIRE |
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