Several known indole compounds are not important precursors of direct mutagenic N-nitroso compounds in green cabbage
| Autor: | A.M. Hissink, S.M. Lodema, H.G.M. Tiedink, Wim M.F. Jongen, L.W. van Broekhoven |
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| Rok vydání: | 1990 |
| Předmět: |
Salmonella typhimurium
Indole test Indole Compound Indoles Chromatography Nitroso Compounds Elution Health Toxicology and Mutagenesis Glucosinolates Tryptophan Brassica Mass spectrometry Gas Chromatography-Mass Spectrometry Gel permeation chromatography chemistry.chemical_compound chemistry Biochemistry Chromatography Gel Genetics Nitrite Molecular Biology Chromatography High Pressure Liquid Mutagens |
| Zdroj: | Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 232:199-207 |
| ISSN: | 0027-5107 |
| Popis: | In this study we investigated the role of indole-3-acetonitrile, indole-3-carbinol, indole and tryptophan in the formation of N -nitroso compounds in green cabbage extracts. Green cabbage extracts were separated by gel permeation chromatography. Fractions were treated with nitrite, tested for mutagenicity and analysed for total N -nitroso. Fractions in which spiked indole-3-acetonitrile, indole-3-carnibol, indole and tryptophan eluted appeared to be low in mutagenic activity and contained relatively small amounts of N -nitroso compounds. To detect indole compounds other than the ones used in the gel permeation chromatography experiments, high-performance liquid chromatography and gas chromatography-mass spectrometry analyses were performed of green cabbage extracts. Indole-3-carboxaldehyde was found to be the most commonly occuring indole compound, but it did not show direct mutagenic activity upon nitrite treatment. Indole-3-acetonitrile was the second most common compound; although it was mutagenic after nitrite treatment, its contribution to the mutagenicity of nitrite-treated green cabbage was roughly estimated to be only 2%. No other indole compounds were detected. From this study we conclude that neither the tested indole compounds nor indole-3-carboxaldehyde play a significant role in the formation of direct mutagenic N -nitroso compounds in nitrate-treated green cabbage extracts. |
| Databáze: | OpenAIRE |
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