Quantification of Protozoa and Viruses from Small Water Volumes
| Autor: | Troy M. Scott, Carol J. Palmer, Helena M. Solo-Gabriele, J. Alfredo Bonilla, Tonya D. Bonilla, Amir M. Abdelzaher, Jerzy Lukasik |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Health
Toxicology and Mutagenesis Cryptosporidium lcsh:Medicine medicine.disease_cause Immunomagnetic separation Polymerase Chain Reaction water quality Article Microbiology medicine Seawater 14. Life underwater Virus quantification Chromatography biology Immunomagnetic Separation enterovirus Giardia lcsh:R Public Health Environmental and Occupational Health biology.organism_classification Poliovirus quantitative PCR Enterovirus Protozoa Sample collection GIARDIA SPP Water Microbiology Filtration |
| Zdroj: | International Journal of Environmental Research and Public Health Volume 12 Issue 7 Pages 7118-7132 International Journal of Environmental Research and Public Health, Vol 12, Iss 7, Pp 7118-7132 (2015) |
| ISSN: | 1660-4601 |
| DOI: | 10.3390/ijerph120707118 |
| Popis: | Large sample volumes are traditionally required for the analysis of waterborne pathogens. The need for large volumes greatly limits the number of samples that can be processed. The goals of this study were to compare extraction and detection procedures for quantifying protozoan parasites and viruses from small volumes of marine water. The intent was to evaluate a logistically simpler method of sample collection and processing that would facilitate direct pathogen measures as part of routine monitoring programs. Samples were collected simultaneously using a bilayer device with protozoa capture by size (top filter) and viruses capture by charge (bottom filter). Protozoan detection technologies utilized for recovery of Cryptosporidium spp. and Giardia spp. were qPCR and the more traditional immunomagnetic separation—IFA-microscopy, while virus (poliovirus) detection was based upon qPCR versus plaque assay. Filters were eluted using reagents consistent with the downstream detection technologies. Results showed higher mean recoveries using traditional detection methods over qPCR for Cryptosporidium (91% vs. 45%) and poliovirus (67% vs. 55%) whereas for Giardia the qPCR-based methods were characterized by higher mean recoveries (41% vs. 28%). Overall mean recoveries are considered high for all detection technologies. Results suggest that simultaneous filtration may be suitable for isolating different classes of pathogens from small marine water volumes. More research is needed to evaluate the suitability of this method for detecting pathogens at low ambient concentration levels. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|