The Role of Salmonella Genomic Island 4 in Metal Tolerance of Salmonella enterica Serovar I 4,[5],12:i:- Pork Outbreak Isolate USDA15WA-1
| Autor: | Shawn M.D. Bearson, Nicholas K. Gabler, Julian Trachsel, Brian W. Brunelle, Brian J. Kerr, Crystal L. Loving, Daniel C. Shippy, Shelby M Curry, Bradley L. Bearson, Sathesh K. Sivasankaran |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Serotype Salmonella Genomic Islands metal tolerance lcsh:QH426-470 Swine Feed additive 030106 microbiology Microbial Sensitivity Tests Biology medicine.disease_cause Serogroup Article Microbiology Disease Outbreaks 03 medical and health sciences Plasmid Genomic island Drug Resistance Multiple Bacterial Genetics medicine Animals Genetics (clinical) mobile genetic element Outbreak Salmonella enterica Antimicrobial biology.organism_classification Drug Resistance Multiple United States Anti-Bacterial Agents Interspersed Repetitive Sequences lcsh:Genetics 030104 developmental biology copper Pork Meat conjugation |
| Zdroj: | Genes, Vol 11, Iss 1291, p 1291 (2020) Genes Volume 11 Issue 11 |
| ISSN: | 2073-4425 |
| Popis: | Multidrug-resistant (MDR resistance to > 3 antimicrobial classes) Salmonella enterica serovar I 4,[5],12:i:- strains were linked to a 2015 foodborne outbreak from pork. Strain USDA15WA-1, associated with the outbreak, harbors an MDR module and the metal tolerance element Salmonella Genomic Island 4 (SGI-4). Characterization of SGI-4 revealed that conjugational transfer of SGI-4 resulted in the mobile genetic element (MGE) replicating as a plasmid or integrating into the chromosome. Tolerance to copper, arsenic, and antimony compounds was increased in Salmonella strains containing SGI-4 compared to strains lacking the MGE. Following Salmonella exposure to copper, RNA-seq transcriptional analysis demonstrated significant differential expression of diverse genes and pathways, including induction of at least 38 metal tolerance genes (copper, arsenic, silver, and mercury). Evaluation of swine administered elevated concentrations of zinc oxide (2000 mg/kg) and copper sulfate (200 mg/kg) as an antimicrobial feed additive (Zn+Cu) in their diet for four weeks prior to and three weeks post-inoculation with serovar I 4,[5],12:i:- indicated that Salmonella shedding levels declined at a slower rate in pigs receiving in-feed Zn+Cu compared to control pigs (no Zn+Cu). The presence of metal tolerance genes in MDR Salmonella serovar I 4,[5],12:i:- may provide benefits for environmental survival or swine colonization in metal-containing settings. |
| Databáze: | OpenAIRE |
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