Characterization of Porcine Urinary Bladder Matrix Hydrogels from Sodium Dodecyl Sulfate Decellularization Method
| Autor: | Huynh-Quang-Dieu Nguyen, Yu-Chuan Weng, Chen-Yu Kao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Scaffold
Polymers and Plastics 02 engineering and technology complex mixtures Article lcsh:QD241-441 03 medical and health sciences chemistry.chemical_compound peracetic acid Tissue engineering lcsh:Organic chemistry Peracetic acid medicine Sodium dodecyl sulfate 030304 developmental biology 0303 health sciences Decellularization Urinary bladder General Chemistry biochemical phenomena metabolism and nutrition 021001 nanoscience & nanotechnology urinary bladder matrix Trustworthiness medicine.anatomical_structure chemistry sodium dodecyl sulfate Self-healing hydrogels decellularization extracellular matrix hydrogel 0210 nano-technology Biomedical engineering |
| Zdroj: | Polymers, Vol 12, Iss 3007, p 3007 (2020) Polymers Volume 12 Issue 12 |
| ISSN: | 2073-4360 |
| Popis: | Urinary bladder matrix (UBM) is one of the most studied extracellular matrixes (ECM) in the tissue engineering field. Although almost all of the UBM hydrogels were prepared by using peracetic acid (PAA), recent studies indicated that PAA was not a trustworthy way to decellularize UBM. A stronger detergent, such as sodium dodecyl sulfate (SDS), may help tackle this issue however, its effects on the hydrogels&rsquo characteristics remain unknown. Therefore, the objective of this study was to develop a more reliable protocol to decellularize UBM, using SDS, and to compare the characteristics of hydrogels obtained from this method to the widely employed technique, using PAA. The results indicated that SDS was superior to PAA in decellularization efficacy. Different decellularization methods led to dissimilar gelation kinetics however, the methods did not affect other hydrogel characteristics in terms of biochemical composition, surface morphology and rheological properties. The SDS-treated hydrogels possessed excellent cytocompatibility in vitro. These results showed that the SDS decellularization method could offer a more stable and safer way to obtain acellular UBM, due to reducing immunogenicity. The hydrogels prepared from this technique had comparable characteristics as those from PAA and could be a potential candidate as a scaffold for tissue remodeling. |
| Databáze: | OpenAIRE |
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