Isolation and Characterization ofeft-1, an Elongation Factor 2-Like Gene on Chromosome III ofCaenorhabditis elegans
| Autor: | E. P. M. Candido, E. N. Ofulue |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Sequence analysis
Molecular Sequence Data Biology Chromosomes Conserved sequence Gene product Peptide Elongation Factor 2 Cricetinae Sequence Homology Nucleic Acid Genetics Animals Amino Acid Sequence Cloning Molecular Molecular Biology Gene Peptide sequence Caenorhabditis elegans Base Sequence Nucleic acid sequence DNA Cell Biology General Medicine Blotting Northern Peptide Elongation Factors biology.organism_classification Molecular biology Elongation factor Blotting Southern Genes Caenorhabditis |
| Zdroj: | DNA and Cell Biology. 11:71-82 |
| ISSN: | 1557-7430 1044-5498 |
| DOI: | 10.1089/dna.1992.11.71 |
| Popis: | A gene (eft-1) encoding an elongation factor 2-like protein was isolated from a region adjacent to the polyubiquitin gene, ubq-1, of Caenorhabditis elegans. Sequence analysis of genomic and cDNA clones revealed that the deduced amino acid sequence of the protein (EFT-1) is 38% identical to that of mammalian and Drosophila elongation factor 2 (EF-2). The entire eft-1 gene is approximately 3.8 kb in length and contains 5 exons separated by short introns of 46-75 bp. The 2,547-bp open reading frame predicts a protein of 849 amino acid residues (calculated Mr, 96,151). Conserved sequences shared among a variety of GTP-binding proteins including EF-2 are found in the amino-terminal third of EFT-1. The carboxy-terminal half contains regions with 40-57% similarity (including conservative changes) with segments characteristic of EF-2 and its prokaryotic homolog, EF-G. However, the histidyl residue target for ADP-ribosylation of EF-2 by diphtheria toxin is replaced by tyrosine in EFT-1. Southern and Northern blot analyses indicate that eft-1 is a single-copy gene that is expressed at all stages of nematode development. Amplification of fragments encoding highly conserved regions of EF-2 using the polymerase chain reaction led to the isolation of a fragment encoding the modifiable histidyl residue and which likely represents part of the C. elegans EF-2 gene (eft-2). This suggests that EFT-1 is not the C. elegans homolog of EF-2, but a closely related protein. |
| Databáze: | OpenAIRE |
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