Helicobacter pylori infection associates with a mucosal downregulation of Ghrelin, negative regulator of Th1-cell responses
| Autor: | Ivan Monteleone, Giovanni Monteleone, Roberta Caruso, Flavio Caprioli, Francesco Pallone, Omero Alessandro Paoluzi, Manfredi Tesauro, Mario Turriziani, Giovanna Del Vecchio Blanco |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_treatment Down-Regulation Peripheral blood mononuclear cell Helicobacter Infections Interferon-gamma Downregulation and upregulation Interferon Gastric mucosa medicine Humans Gastritis Helicobacter pylori Th1 cells ghrelin Female Gastric Mucosa Ghrelin Interleukin-2 Middle Aged Th1 Cells Th17 Cells Lamina propria Settore MED/12 - Gastroenterologia biology Gastroenterology Interleukin General Medicine biology.organism_classification Infectious Diseases medicine.anatomical_structure Cytokine Immunology medicine.drug |
| Popis: | Background Helicobacter pylori (Hp)-related gastritis is characterized by a predominant T helper (Th)1/Th17 cell immunity. Ghrelin (GR) has immunoregulatory properties and inhibits experimental Th cell-dependent pathology. Aims To evaluate whether Hp infection associates with changes in GR expression and whether GR negatively regulates Th1/Th17 cytokines during Hp infection. Methods GR expression was evaluated by real-time PCR in gastric biopsies taken from Hp-infected and Hp-uninfected patients and in gastric biopsies of Hp-negative subjects cultured with or without H. pylori culture supernatant. To examine whether GR regulates Hp-induced cytokine production, H. pylori-infected gastric biopsies were stimulated with GR, and interleukin (IL)-12, interferon (IFN)-γ and IL-4 transcripts were evaluated by real-time PCR. IL-12 and IFN-γ were also analyzed in lamina propria mononuclear cells (LPMCs) extracted from Hp-infected gastric biopsies and cultured with GR. Results GR RNA transcripts were reduced in biopsies from Hp-infected patients. Treatment of Hp-negative gastric biopsies with Hp culture supernatant reduced GR RNA expression. GR dose-dependently inhibited RNA expression of IL-12 and IFN-γ but not IL-4 in ex vivo cultures of mucosal explants and in cultures of gastric LPMCs from Hp-positive patients. Conclusions GR is downregulated in the gastric mucosa of H. pylori-infected patients. Such a defect could contribute to sustain the ongoing Th1-cell response. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|