Identification of non-invasive biomarkers for predicting the radiosensitivity of nasopharyngeal carcinoma from serum microRNAs
| Autor: | Kai-Guo Li, Shi-Ting Huang, Zhong-Guo Liang, Xiao-Hui Yang, Fang Su, Ling Li, Ruiling Ning, Xiao-Dong Zhu, Song Qu, Fanyan Zeng |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Oncology Adult Male medicine.medical_specialty China Microarray lcsh:Medicine Predictive markers Real-Time Polymerase Chain Reaction Radiation Tolerance Article Biomarkers Pharmacological 03 medical and health sciences 0302 clinical medicine Internal medicine Radioresistance microRNA medicine Biomarkers Tumor Humans Radiosensitivity lcsh:Science Head and neck cancer Aged Aged 80 and over Multidisciplinary Nasopharyngeal Carcinoma Receiver operating characteristic business.industry Gene Expression Profiling lcsh:R Carcinoma Nasopharyngeal Neoplasms Middle Aged medicine.disease Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology Real-time polymerase chain reaction Nasopharyngeal carcinoma ROC Curve 030220 oncology & carcinogenesis Inclusion and exclusion criteria miRNAs lcsh:Q Female business |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-9 (2020) |
| ISSN: | 2045-2322 |
| Popis: | Serum microRNAs (miRNAs) have been reported as novel biomarkers for various diseases. But circulating biomarkers for predicting the radiosensitivity of nasopharyngeal carcinoma (NPC) have not been used in clinical practice. To screen out of differently expressed serum miRNAs from NPC patients with different radiosensitivity may be helpful for its individual therapy. NPC patients with different radiosensitivity were enrolled according to the inclusion and exclusion criteria. RNA was isolated from serum of these NPC patients before treatment. We investigated the differential miRNA expression profiles using microarray test (GSE139164), and the candidate miRNAs were validated by reverse transcription-quantitative real time polymerase chain reaction (RT-qPCR) experiments. Receiver operating characteristic (ROC) analysis has been applied to estimate the diagnostic value. In this study, 37 serum-specific miRNAs were screened out from 12 NPC patients with different radiosensitivity by microarray test. Furthermore, RT-qPCR analysis confirmed that hsa-miR-1281 and hsa-miR-6732-3p were significantly downregulated in the serum of radioresistant NPC patients (P |
| Databáze: | OpenAIRE |
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