Identification, expression profiling and localization of thoc in common carp ovary: Influence of thoc3-siRNA transient silencing
Autor: | Yugantak Raj Gupta, Balasubramanian Senthilkumaran |
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Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Fish Proteins endocrine system Carps Sex Differentiation THO complex medicine.medical_treatment In situ hybridization Biology Oogenesis Evolution Molecular 03 medical and health sciences 0302 clinical medicine Ovarian Follicle Genetics medicine Gene silencing Animals RNA Small Interfering Transcription factor Cell Nucleus Messenger RNA Estradiol Growth factor Gene Expression Profiling Ovary Gene Expression Regulation Developmental Nuclear Proteins General Medicine Cell biology Gene expression profiling 030104 developmental biology 030220 oncology & carcinogenesis Female |
Zdroj: | Gene. 732 |
ISSN: | 1879-0038 |
Popis: | THO complex is a multisubunit family with a function in transcription and mRNA export. In the present study, transcripts of THO complex (thoc) were identified in developing ovary of common carp and their role during ovarian development and growth has been characterized for the first time in a teleost using expression profiling and transient siRNA silencing. Thoc expression revealed a spatiotemporal pattern in the gonads with high levels at 120 days post-hatch, with moderately high levels thereafter. In situ hybridization and immunohistochemical localization revealed the presence of thoc3 in follicular layer of stage-III/IV oocytes. High levels of thoc3, thoc5, and thoc7 genes in the follicular layer suggest a possible role in ovarian growth. Reduced levels of serum estradiol-17β and 17α, 20β-dihydroxypregn-4-en-3-one after thoc3 transient silencing indicated differential action on steroidogenic enzyme, transcription factor, and growth factor genes. Furthermore, transient silencing of thoc3, in vivo and in vitro, downregulated ad4bp/sf1, amh, cyp19a1a, foxl2, hsd3b, hsd11b1, hsd20b, hsd17b1, rspo1, and vtg. Incidentally, gdf9 and igf1 were upregulated, while no change was seen in esr1/2, nanos, and vasa. These observations imply that thoc3 seems to regulate ovarian function including steroidogenesis, either directly or indirectly. |
Databáze: | OpenAIRE |
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