Controlled release microparticles as a single dose hepatitis B vaccine: evaluation of immunogenicity in mice
| Autor: | Chang Yi Wang, Manmohan Singh, Derek T. O'Hagan, Wayne C. Koff, Tim Zamb, J.P. McGee, Xuan-Mao Li |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
HBsAg
medicine.drug_class chemical and pharmacologic phenomena Monoclonal antibody Microbiology Antigen-Antibody Reactions Mice chemistry.chemical_compound Antigen Animals Medicine Hepatitis B Vaccines Particle Size Microparticle Immunization Schedule Vaccines Synthetic Hepatitis B Surface Antigens General Veterinary General Immunology and Microbiology biology Alum business.industry Immunogenicity Public Health Environmental and Occupational Health Antibodies Monoclonal biology.organism_classification Controlled release Molecular biology Biodegradation Environmental Infectious Diseases chemistry Hepadnaviridae Evaluation Studies as Topic Delayed-Action Preparations Molecular Medicine business |
| Zdroj: | Vaccine. 15:475-481 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/s0264-410x(97)00225-9 |
| Popis: | Hepatitis B surface antigen (HBsAg) was encapsulated in microparticles prepared from polylactide-co-glycolide (PLG) and polylactide (PLA) polymers using a solvent evaporation process. The immunoreactivity of the entrapped antigen was investigated by SDS-PAGE and Western blot. The microencapsulation process was modified to obtain both small (10 microns) and large microparticles (10-100microns). 80% of the antigen was encapsulated. Various combinations of small and large microparticles with controlled release characteristics were investigated in CD1 mice. Groups of animals were immunized with 30 micrograms equivalent of HBsAg in microparticles per animals. The control group received, three injections of 10 micrograms of HBsAg on alum at 0, 1 and 6 months. Results indicated that a single injection of HBsAg in microparticles could maintain the antibody response at a level comparable to the three-injection alum schedule for at least 1 year. An in vitro inhibition assay was developed to demonstrate that antigen-antibody reactivity were comparable for the microparticle immunized mice and the alum immunized mice. A competition assay with a monoclonal antibody specific for the neutralizing epitope of HBsAg demonstrated comparable binding for the sera from the microparticle and alum immunized mice. |
| Databáze: | OpenAIRE |
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