Early death of Japanese encephalitis virus-infected mice administered a neutralizing cross-reactive monoclonal antibody against glycoprotein E
| Autor: | V J Lad, A K Gupta, A A Koshy |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
medicine.drug_class
viruses Biology Cross Reactions Monoclonal antibody Virus Mice Viral Envelope Proteins Virology medicine Animals Encephalitis Japanese chemistry.chemical_classification Encephalitis Virus Japanese Mice Inbred BALB C Membrane Glycoproteins Antibodies Monoclonal Brain Biological activity General Medicine Japanese encephalitis Hemagglutination Inhibition Tests medicine.disease Antibodies Neutralizing Titer Infectious Diseases chemistry biology.protein Antibody Glycoprotein Encephalitis |
| Zdroj: | Acta virologica. 53(3) |
| ISSN: | 0001-723X |
| Popis: | UNLABELLED In the present study, the effect of two haemagglutination-inhibition (HAI)-negative auto-reactive (NHA-1 and NHA-2) monoclonal antibodies (MAbs) against glycoprotein E (gpE) of Japanese encephalitis virus (JEV) administered 1 day before or 2 days after intracerebral (i.c.) inoculation of JEV was studied in mice. Of the two MAbs that cross-reacted with West Nile virus (WNV) and histones, the first one (NHA-1) neutralized JEV, while the second one was non-neutralizing. NHA-1 MAb given intraperitoneally (i.p.) 1 day before virus infection induced early death by about 2 days in comparison to controls, whereas mice administered HAI-positive anti-gpE JEV specific MAbs (Hs-1 or Hs-4) were invariably protected. In contrast, MAb NHA-2 failed to produce any effect in mice. Since the similar virus titers were recorded in the brains of experimental and control infected mice, the present results indicated a modification of the biological activity of JEV by the pre-existing MAb NHA-1 that might be leading to an early death of mice. KEYWORDS Japanese encephalitis virus; neutralizing cross-reactive monoclonal antibody. |
| Databáze: | OpenAIRE |
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