The Biocompatibility of Bone Cements: Progress in Methodological Approach
| Autor: | Gian Mario Micheloni, Daniele Degl'Innocenti, Franco Lavini, Giampaolo Morbioli, Matteo Cengarle, Paolo Bernardi, Andrea Sbarbati, Bruno Magnan, Carlo Dall'Oca, Tommaso Maluta |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
plymethylmetacrilate
calcium phosphate cement osteointegration biocompatibility environmental scanning electron microscopy Calcium Phosphates Histology Materials science Biocompatibility Scanning electron microscope 0206 medical engineering Biophysics Environmental Scanning Electron Microscopy 02 engineering and technology Osseointegration 03 medical and health sciences LR-white resin 0302 clinical medicine Microscopy Materials Testing Technical Note Animals Polymethyl Methacrylate New zealand white lcsh:QH301-705.5 Environmental scanning electron microscope Cement Bone Cements Cell Biology Plymethylmetacrilate 020601 biomedical engineering Magnetic Resonance Imaging Key words: Plymethylmetacrilate lcsh:Biology (General) 030220 oncology & carcinogenesis Bone Substitutes Microscopy Electron Scanning Rabbits Biomedical engineering Follow-Up Studies |
| Zdroj: | European Journal of Histochemistry : EJH European Journal of Histochemistry, Vol 61, Iss 2 (2017) |
| ISSN: | 2038-8306 1121-760X |
| Popis: | The ideal bone graft substitute should have certain properties and there are many studies dealing with mixture of polymethylmetacrilate (PMMA) and β-tricalciumphospate (β-TCP) presenting the best characteristics of both. Scanning Electron Microscopy (SEM), for ultra-structural data, resulted a very reliable in vivo model to better understand the bioactivity of a cement and to properly evaluate its suitability for a particular purpose. The present study aims to further improve the knowledge on osteointegration development, using both parameters obtained with the Environmental Scanning Electron Microscopy (ESEM) and focused histological examination. Two hybrid bone graft substitute were designed among ceramic and polymer-based bone graft substitutes. Based on β-TCP granules sizes, they were created with theoretical different osteoconductive properties. An acrylic standard cement was chosen as control. Cements were implanted in twelve New Zealand White (NZW) rabbits, which were sacrificed at 1, 2, 3, 6, 9 and 12 months after cement implantation. Histological samples were prepared with an infiltration process of LR white resin and then specimens were studied by X-rays, histology and Environmental Scanning Electron Microscopy (ESEM). Comparing the resulting data, it was possible to follow osteointegration’s various developments resulting from different sizes of β-TCP granules. In this paper, we show that this evaluation process, together with ESEM, provides further important information that allows to follow any osteointegration at every stage of develop. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|