Adult stem cell lineage tracing and deep tissue imaging
| Autor: | Bon-Kyoung Koo, Amanda Andersson-Rolf, Juergen Fink |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Computational biology
Biology Tracing Lineage tracing Biochemistry Advanced imaging 03 medical and health sciences 0302 clinical medicine Live cell imaging Intravital imaging Animals Homeostasis Humans Cell Lineage Progenitor cell Molecular Biology 030304 developmental biology Adult stem cells 0303 health sciences Tissue clearing Optical Imaging General Medicine Intravital Imaging Invited Mini Review Cell biology Multicellular organism Cell Tracking 030217 neurology & neurosurgery Adult stem cell |
| Zdroj: | BMB Reports |
| ISSN: | 1976-6696 |
| DOI: | 10.5483/bmbrep.2015.48.12.249 |
| Popis: | Lineage tracing is a widely used method for understanding cellular dynamics in multicellular organisms during processes such as development, adult tissue maintenance, injury repair and tumorigenesis. Advances in tracing or tracking methods, from light microscopy-based live cell tracking to fluorescent label-tracing with two-photon microscopy, together with emerging tissue clearing strategies and intravital imaging approaches have enabled scientists to decipher adult stem and progenitor cell properties in various tissues and in a wide variety of biological processes. Although technical advances have enabled time-controlled genetic labeling and simultaneous live imaging, a number of obstacles still need to be overcome. In this review, we aim to provide an in-depth description of the traditional use of lineage tracing as well as current strategies and upcoming new methods of labeling and imaging. [BMB Reports 2015; 48(12): 655-667] |
| Databáze: | OpenAIRE |
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