Distance-dependent gradient in NMDAR-driven spine calcium signals along tapering dendrites
| Autor: | Gema Vizcay-Barrena, Mark Rigby, Cian O'Donnell, Roland A. Fleck, Federico W. Grillo, Juan Burrone, Andrew S. Lowe, Alison S. Walker, Guilherme Neves, Rachel E. Jackson |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Dendritic spine N-Methylaspartate Dendritic Spines Primary Cell Culture chemistry.chemical_element Hippocampus Gene Expression Dendrite Calcium Biology Hippocampal formation Receptors N-Methyl-D-Aspartate Rats Sprague-Dawley 03 medical and health sciences Mice 0302 clinical medicine Pregnancy medicine Journal Article Animals Calcium Signaling Dendritic spike Multidisciplinary Neuronal Plasticity Pyramidal Cells Microtomy Embryo Mammalian Rats Mice Inbred C57BL Microscopy Electron 030104 developmental biology medicine.anatomical_structure chemistry nervous system NMDA PNAS Plus Synaptic plasticity Synapses NMDA receptor Female Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Walker, A S, Neves, G, Grillo, F, Jackson, R E, Rigby, M, O'Donnell, C, Lowe, A S, Vizcay-Barrena, G, Fleck, R A & Burrone, J 2017, ' Distance-dependent gradient in NMDAR-driven spine calcium signals along tapering dendrites ', Proceedings of the National Academy of Sciences of the United States of America, vol. 114, no. 10, pp. 1986-1995 . https://doi.org/10.1073/pnas.1607462114 Walker, A S, Neves, G, Grillo, F, Jackson, R E, Rigby, M, O'Donnell, C, Lowe, A S, Vizcay-Barrena, G, Fleck, R A & Burrone, J 2017, ' Distance-dependent gradient in NMDAR-driven spine calcium signals along tapering dendrites ', Proceedings of the National Academy of Sciences of the United States of America, vol. 114, no. 10, pp. E1986-E1995 . https://doi.org/10.1073/pnas.1607462114 |
| ISSN: | 1091-6490 |
| DOI: | 10.1073/pnas.1607462114 |
| Popis: | Neurons receive a multitude of synaptic inputs along their dendritic arbor, but how this highly heterogeneous population of synaptic compartments is spatially organized remains unclear. By measuring N-methyl-d-aspartic acid receptor (NMDAR)-driven calcium responses in single spines, we provide a spatial map of synaptic calcium signals along dendritic arbors of hippocampal neurons and relate this to measures of synapse structure. We find that quantal NMDAR calcium signals increase in amplitude as they approach a thinning dendritic tip end. Based on a compartmental model of spine calcium dynamics, we propose that this biased distribution in calcium signals is governed by a gradual, distance-dependent decline in spine size, which we visualized using serial block-face scanning electron microscopy. Our data describe a cell-autonomous feature of principal neurons, where tapering dendrites show an inverse distribution of spine size and NMDAR-driven calcium signals along dendritic trees, with important implications for synaptic plasticity rules and spine function. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|