Bioconjugation and stabilisation of biomolecules in biosensors
| Autor: | Guido A. Drago, Susana Liébana |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
bioconjugation
Nanotechnology Biosensing Techniques 02 engineering and technology 01 natural sciences Biochemistry Article screen-printed electrodes Protein stability Electrochemical biosensor Operational stability Molecular Biology electrochemical biosensors magnetic particles chemistry.chemical_classification Bioconjugation Biomolecule 010401 analytical chemistry 021001 nanoscience & nanotechnology 0104 chemical sciences Immobilized Proteins protein stability chemistry 0210 nano-technology Biosensor bioreceptor immobilisation |
| Zdroj: | Essays in Biochemistry |
| ISSN: | 1744-1358 0071-1365 |
| Popis: | Suitable bioconjugation strategies and stabilisation of biomolecules on electrodes is essential for the development of novel and commercially viable biosensors. In the present review, the functional groups that comprise the selectable targets for practical bioconjugation methods are discussed. We focus on describing the most common immobilisation techniques used in biosensor construction, which are classified into irreversible and reversible methods. Concerning the stability of proteins, the two main types of stability may be defined as (i) storage or shelf stability, and (ii) operational stability. Both types of stability are explained, as well as the introduction of an electrophoretic technique for predicting protein–polymer interactions. In addition, solution and dry stabilisation as well as stabilisation using the covalent immobilisation of proteins are discussed including possible factors that influence stability. Finally, the integration of nanomaterials, such as magnetic particles, with protein immobilisation is discussed in relation to protein stability studies. |
| Databáze: | OpenAIRE |
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