Vascular endocan is preferentially expressed in tumor endothelium

Autor: Shu-Ching Shih, Xianjin Yi, William C. Aird, Kiichiro Yano, Md. Ruhul Abid
Rok vydání: 2006
Předmět:
Vascular Endothelial Growth Factor A
Endothelium
Mice
Inbred Strains
In situ hybridization
Biology
Biochemistry
Umbilical vein
Mice
chemistry.chemical_compound
Cell Line
Tumor
von Willebrand Factor
medicine
Animals
Humans
RNA
Messenger
Northern blot
Cells
Cultured
In Situ Hybridization
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Profiling
Forkhead Box Protein O3
Endothelial Cells
Gene Expression Regulation
Developmental
Forkhead Transcription Factors
Neoplasms
Experimental
Cell Biology
Blotting
Northern
Embryo
Mammalian
Molecular biology
Neoplasm Proteins
Rats
Platelet Endothelial Cell Adhesion Molecule-1
Vascular endothelial growth factor
Blot
Endothelial stem cell
medicine.anatomical_structure
Gene Expression Regulation
chemistry
Cell culture
Culture Media
Conditioned
Immunology
Proteoglycans
Endothelium
Vascular
Cardiology and Cardiovascular Medicine
Proto-Oncogene Proteins c-akt
Zdroj: Microvascular Research. 72:136-145
ISSN: 0026-2862
DOI: 10.1016/j.mvr.2006.05.010
Popis: Endothelial cell phenotypes are differentially regulated between different sites of the vascular tree. We tested the hypothesis that endocan, a novel soluble dermatan sulfate proteoglycan, is differentially expressed in the intact endothelium and that site-specific expression is mediated by signals in the local microenvironment. Using a combination of Northern blot analyses, Taqman RT-PCR, and in situ hybridizations, endocan was shown to be preferentially expressed in the endothelial lining of tumor xenografts, including human non-small cell lung cancer, rat glioma, and human renal cell carcinoma. In contrast, endocan mRNA was expressed at low levels in embryos between E4.5 and E18.5. Under in vitro conditions, endocan expression in human umbilical vein endothelial cells (HUVEC) was upregulated by tumor cell-conditioned medium, an effect that was inhibited by the addition of neutralizing antibody to vascular endothelial growth factor (VEGF). Moreover, treatment of HUVEC with VEGF resulted in a dose- and time-dependent increase in endocan mRNA. The results suggest that endocan is preferentially expressed in tumor endothelium in vivo and that its expression is regulated by tumor-derived factors.
Databáze: OpenAIRE
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