MMP 1 circulating levels and promoter polymorphism in risk prediction of coronary artery disease in asymptomatic first degree relatives
| Autor: | Nivas Shyamala, Pratibha Nallari, Sanjib Kumar Sahu, Anuradha Cingeetham, Kishore Kumar Gundapaneni, Mrudula Spurthi Kondapalli, Surekha Rani Hanumanth, Rajesh Kumar Galimudi, Srilatha Reddy Gantala, Chiranjeevi Padala, Altaf Ali |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
Male 0301 basic medicine medicine.medical_specialty Genotype MMP1 Coronary Artery Disease 030204 cardiovascular system & hematology Biology Matrix metalloproteinase Asymptomatic Gastroenterology Coronary artery disease 03 medical and health sciences 0302 clinical medicine Internal medicine Genetics medicine Humans Genetic Predisposition to Disease Allele First-degree relatives Promoter Regions Genetic Alleles Polymorphism Genetic General Medicine Middle Aged medicine.disease 030104 developmental biology Female Matrix Metalloproteinase 1 Restriction fragment length polymorphism medicine.symptom |
| Zdroj: | Gene. 595:115-120 |
| ISSN: | 0378-1119 |
| Popis: | Coronary artery disease (CAD) remains to be the prominent health problem in India, and its incidence is growing in developing countries as well. Matrix metalloproteinase 1 (MMP 1) is highly expressed in disruption-prone shoulder regions of the fibrous plaques. The present study aims to investigate association of MMP 1 gene polymorphisms (− 1607 1G/2G) and serum circulating levels with CAD. The study includes 300 CAD patients, 100 FDRS, and 300 controls. ELISA and PCR-RFLP were performed to determine MMP 1 serum levels and genotypes respectively. MMP1 levels were high in CAD patients, followed by FDRS compared to controls (2.15 ± 1.2 ng/ml; 1.46 ± 1.04 ng/ml and 0.96 ± 0.53 ng/ml) respectively. ROC analysis showed the AUC at 95% CI of serum MMP-1 to be 0.83 and 0.73–0.94, respectively. The optimal cut-off point (sensitivity; specificity) of serum MMP 1 was > 1.5 ng/ml (0.74; 0.90). The 2G/2G genotype was associated with high MMP 1 circulating levels in CAD patients, and a similar trend was observed in FDRS and controls. The pre-mRNA secondary structure of the 2G allele is much more stable than 1G allele. Our results suggest MMP 1 serum levels and polymorphism as potential independent prognostic markers for future cardiovascular events. These may also help to stratify CAD patients and to identify susceptibility for CAD in asymptomatic healthy FDRS. |
| Databáze: | OpenAIRE |
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