Production of electricity and reduction of high-fat diet-induced IL-6 by glucose fermentation of Leuconostoc mesenteroides
| Autor: | Tsung Hsien Chuang, Albert Jackson Yang, Adelia Riezka Rahim, John Jackson Yang, Chun Ming Huang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_treatment Biophysics Administration Oral Leuconostoc mesenteroides Acetates Diet High-Fat Furfural Biochemistry Article Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Electricity Oral administration medicine Animals Furaldehyde Food science Interleukin 6 Molecular Biology Incubation IL-6 biology Interleukin-6 L. mesenteroides food and beverages Cell Biology biology.organism_classification Glucose 030104 developmental biology Cytokine chemistry Electrogenic 030220 oncology & carcinogenesis Fermentation Food Microbiology biology.protein Female Bacteria |
| Zdroj: | Biochemical and Biophysical Research Communications |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2020.09.105 |
| Popis: | Electrogenic bacteria can mediate electron transfer to conserve energy and promote growth. To examine bacterial electrogenicity, an L. mesenteroides EH-1 strain was cultured in rich media in the presence and absence of 2% glucose. After 12 h incubation, glucose triggered fermentation of L. mesenteroides EH-1 to produce >10 mmol/l acetate and elicit electricity measured by voltage changes. The electricity production was mediated by glucose fermentation since pre-treatment of L. mesenteroides EH-1 with furfural, a fermentation inhibitor, completely diminished the voltage increases. The deficiency of furfural pre-treated L. mesenteroides EH-1 in electricity production can be restored by the external addition of acetate into the bacterial culture, suggesting the function of acetate as an electron donor. Oral administration of HFD-fed mice with L. mesenteroides EH-1 in the presence or absence of glucose significantly attenuated the high level of pro-inflammatory IL-6 cytokine in blood. Bacterial electricity can be elicited by fermentation. Supplementation of fermenting and electrogenic L. mesenteroides EH-1 may provide a novel approach for the reduction of pro-inflammatory IL-6 cytokine that increased in chronic inflammation, autoimmune diseases, cancers, and infections. Highlights • Electricity production of L. mesenteroides EH-1 was mediated by glucose fermentation. • Pre-treatment of L. mesenteroides EH-1 with furfural completely diminished electricity. • Acetate restore L. mesenteroides EH-1 electricity production pre-treated with Furfural. • Administration of L. mesenteroides EH-1 significantly decreased the IL-6 in blood. |
| Databáze: | OpenAIRE |
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