In vitro evaluation of immunobiological activity of simple mannolipids
| Autor: | Miroslav Panik, Soňa Jantová, Monika Poláková, Lucia Paulovičová, Ema Paulovičová |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cell Survival Antineoplastic Agents Toxicology Eye Models Biological Proinflammatory cytokine Cell Line HeLa Immunomodulation 03 medical and health sciences Mice 0302 clinical medicine Macrophage Animals Humans Immunologic Factors Cytotoxicity Cell Proliferation Skin biology Chemistry General Medicine biology.organism_classification In vitro 030104 developmental biology Cell culture 030220 oncology & carcinogenesis Mannosides Cancer cell Cancer research Cytokines Tumor necrosis factor alpha |
| Zdroj: | Toxicology in vitro : an international journal published in association with BIBRA. 70 |
| ISSN: | 1879-3177 |
| Popis: | Immunomodulation, cytotoxicity and anti-cancer activity of selected amphiphilic non-ionic (thio)alkyl α-D-mannosides (with aglycone of C6-C12) were investigated in vitro in human cervix epitheloid carcinoma cell line HeLa, murine melanoma cancer cells B16, murine lymphocytic leukemia cell line L1210, murine fibroblast cell line NIH 3 T3 and murine macrophage cell line RAW 264.7. Toxicological studies revealed structure-dependent immunobiological effectivity based on a tight interaction with relevant cells. The results demonstrated diverse immunomodulation of macrophage cell-line RAW264.7 proliferation and production of Th1 and Th2 cytokines, and induction of pro-inflammatory interleukins IL-1α, TNFα, IL-6, IL-12 and IL-17 and anti-inflammatory IL-10 following (thio)alkyl α-D-mannosides 24 and 48 h exposure. Direct application of alkyl mannosides MOC10 and MOC12 and their thio analogues MSC10 and MSC12 in reconstructed human EpiDerm™ and MOC12 and MSC12 in EpiOcular™ model assays for dermal and ocular irritation together with quantification of human proinflammatory cytokines IL-1α, TNFα, IL-6 and IL-8 culture media release was used to ascertain toxicological safety. |
| Databáze: | OpenAIRE |
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