Tissue Engineering of Cementum/Periodontal-Ligament Complex Using a Novel Three-Dimensional Pellet Cultivation System for Human Periodontal Ligament Stem Cells
| Autor: | Zhenhua Yang, Zhu Lin, Na Huo, Dandan Ma, Chun Han, Yun-Fei Zhang, Xiaojun Zhang, Yinxiong Wang, Fang Jin, Yan Jin |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Bone sialoprotein
Ceramics Periodontal ligament stem cells Periodontal Ligament Cementoblast Cell Culture Techniques Biomedical Engineering Medicine (miscellaneous) Bioengineering Cell Separation Bone and Bones Rats Sprague-Dawley Extracellular matrix Mice stomatognathic system Tissue engineering medicine Animals Humans Periodontal fiber Cementum Dental Cementum Staining and Labeling Tissue Engineering biology Chemistry Stem Cells Tooth Germ Ascorbic acid Rats Cell biology medicine.anatomical_structure Dentin biology.protein Cattle Stem Cell Transplantation Biomedical engineering |
| Zdroj: | Tissue Engineering Part C: Methods. 15:571-581 |
| ISSN: | 1937-3392 1937-3384 |
| DOI: | 10.1089/ten.tec.2008.0561 |
| Popis: | Limitations of conventional regeneration modalities underscore the necessity of recapitulating development for periodontal tissue engineering. In this study, we proposed a novel three-dimensional pellet cultivation system for periodontal ligament stem cells (PDLSCs) to recreate the biological microenvironment similar to those of a regenerative milieu. Monodispersed human PDLSCs were cultured in medium with ascorbic acid and conditioned medium from developing apical tooth germ cells and were subsequently harvested from culture plate as a contiguous cell sheet with abundant extracellular matrix. The detached cell-matrix membrane spontaneously contracted to produce a single-cell pellet. The PDLSCs embedded within this cell-matrix complex exhibited several phenotypic characteristics of cementoblast lineages, as indicated by upregulated alkaline phosphatase activity, accelerated mineralization, and the expression of bone sialoprotein and osteocalcin genes. When this PDLSC pellets were transplanted into immunocompromised mice, a regular aligned cementum/PDL-like complex was formed. These results suggest that the combination of apical tooth germ cell-conditioned medium and endogenous extracellular matrix could maximally mimic the microenvironment of root/periodontal tissue development and enhance the reconstruction of physiological architecture of a cementum/PDL-like complex in a tissue-mimicking way; on the other hand, such PDLSC pellet may also be a promising alternative to promote periodontal defect repair for future clinical applications. |
| Databáze: | OpenAIRE |
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