Chimeric Stimuli-Responsive Liposomes as Nanocarriers for the Delivery of the Anti-Glioma Agent TRAM-34

Autor: Jakub Kajdanek, Stefano Garofalo, Łucja Balcerzak, Katarzyna Milowska, Natassa Pippa, Maria Bryszewska, Evangelia Sereti, Maksim Ionov, Cristina Limatola, Stergios Pispas, Marta Kędzierska, Costas Demetzos, Varvara Chrysostomou, Konstantinos Dimas, Nikolaos Naziris
Přispěvatelé: Laboratory of Microscopic Imaging and Specialized Biological Techniques, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
Polymers
Apoptosis
02 engineering and technology
Drug Delivery Systems
Tumor Cells
Cultured
Biology (General)
Chimeric liposomes
Drug delivery
Functional
Glioblastoma
PH-responsive
TRAM-34
Cell Proliferation
Drug Carriers
Glioma
Humans
Hydrogen-Ion Concentration
Liposomes
Nanoparticles
Pyrazoles
Spectroscopy
pH-responsive
Liposome
Cultured
Chemistry
General Medicine
021001 nanoscience & nanotechnology
Computer Science Applications
Tumor Cells
0210 nano-technology
Biocompatibility
QH301-705.5
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
In vivo
medicine
Physical and Theoretical Chemistry
Molecular Biology
QD1-999
chimeric liposomes
Organic Chemistry
glioblastoma
medicine.disease
In vitro
functional
030104 developmental biology
Nanotoxicology
drug delivery
Cancer research
Nanocarriers
Zdroj: International Journal of Molecular Sciences, Vol 22, Iss 6271, p 6271 (2021)
International Journal of Molecular Sciences
Volume 22
Issue 12
ISSN: 1661-6596
1422-0067
Popis: Nanocarriers are delivery platforms of drugs, peptides, nucleic acids and other therapeutic molecules that are indicated for severe human diseases. Gliomas are the most frequent type of brain tumor, with glioblastoma being the most common and malignant type. The current state of glioma treatment requires innovative approaches that will lead to efficient and safe therapies. Advanced nanosystems and stimuli-responsive materials are available and well-studied technologies that may contribute to this effort. The present study deals with the development of functional chimeric nanocarriers composed of a phospholipid and a diblock copolymer, for the incorporation, delivery and pH-responsive release of the antiglioma agent TRAM-34 inside glioblastoma cells. Nanocarrier analysis included light scattering, protein incubation and electron microscopy, and fluorescence anisotropy and thermal analysis techniques were also applied. Biological assays were carried out in order to evaluate the nanocarrier nanotoxicity in vitro and in vivo, as well as to evaluate antiglioma activity. The nanosystems were able to successfully manifest functional properties under pH conditions, and their biocompatibility and cellular internalization were also evident. The chimeric nanoplatforms presented herein have shown promise for biomedical applications so far and should be further studied in terms of their ability to deliver TRAM-34 and other therapeutic molecules to glioblastoma cells.
Databáze: OpenAIRE
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