Preparation and characterization of multilayered hydroxyapatite/silk fibroin film
| Autor: | Nobuhiro Nagai, Rikako Kino, Junzo Tanaka, Toshiyuki Ikoma, Masanobu Munekata, Shunji Yunoki, Tetsuo Asakura |
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| Rok vydání: | 2007 |
| Předmět: |
Materials science
Biocompatibility Simulated body fluid Composite number Silk Fibroin Biocompatible Materials Bioengineering Applied Microbiology and Biotechnology law.invention Mice Coated Materials Biocompatible law Tensile Strength Ultimate tensile strength Polymer chemistry Lamination Cell Adhesion Animals Guided Tissue Regeneration Membranes Artificial 3T3 Cells Alkaline Phosphatase Bombyx Biomechanical Phenomena Durapatite Membrane SILK Chemical engineering Microscopy Electron Scanning Fibroins Biotechnology |
| Zdroj: | Journal of Bioscience and Bioengineering. 103:514-520 |
| ISSN: | 1389-1723 |
| DOI: | 10.1263/jbb.103.514 |
| Popis: | We prepared multilayered films consisting of silk fibroin (SF) and hydroxyapatite (HAp) by alternating lamination using untreated SF and HAp-deposited SF films. Untreated SF films were prepared from a regenerated SF solution by air drying. HAp-deposited SF films were prepared by soaking methanol-treated SF films containing >5 wt% CaCl2 in a simulated body fluid with the ion concentration 1.5-fold higher than that of the standard one. The multilayered HAp/SF films had HAp layers with approximate thicknesses of 3-5 microm and SF layers with thicknesses of 40-70 microm. The bonding strength between the SF and HAp layers was significantly affected by temperature and compression time under the lamination method. The optimal conditions for achieving the maximum T-peel strength and beta-sheet contents were determined to be 130 degrees C for 4 min. The Young's modulus of the multilayered films (133.4 MPa) was higher than that of the films consisting of SF alone (92.5 MPa) under swollen conditions. The biocompatibility of the HAp-deposited SF films was analyzed by culturing of osteoblasts (MC3T3-E1) on a film. The results indicate that HAp-deposited SF films and SF films show similar degrees of cell adhesion and alkaline phosphatase activities. |
| Databáze: | OpenAIRE |
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