Visualization of mucosal field in HPV positive and negative oropharyngeal squamous cell carcinomas: combined genomic and radiology based 3D model

Autor: Eva Orosz, Andras Burian, Zalán Piski, Imre Gerlinger, Szabolcs Bellyei, Nerina Petrevszky, Krisztian Molnar, Arnold Tóth, Katalin Gombos, István Szanyi, Bálint Kaszás, David Csonka, Krisztina Kalacs, István Háber
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Male
Pathology
Cell
lcsh:Medicine
0302 clinical medicine
Genetics research
Medicine
Sampling (medicine)
Stage (cooking)
Child
lcsh:Science
Papillomaviridae
Regulation of gene expression
Multidisciplinary
medicine.diagnostic_test
Molecular medicine
Genomics
Middle Aged
Prognosis
Gene Expression Regulation
Neoplastic
Survival Rate
Oropharyngeal Neoplasms
medicine.anatomical_structure
030220 oncology & carcinogenesis
Child
Preschool
Carcinoma
Squamous Cell
Female
Mouth Neoplasms
Adult
medicine.medical_specialty
Adolescent
Article
03 medical and health sciences
Young Adult
Imaging
Three-Dimensional
Biopsy
Carcinoma
Biomarkers
Tumor
Humans
Author Correction
Survival rate
Aged
Mucous Membrane
business.industry
Papillomavirus Infections
lcsh:R
Infant
Newborn
Cancer
Infant
medicine.disease
MicroRNAs
030104 developmental biology
lcsh:Q
business
Tomography
X-Ray Computed
Follow-Up Studies
Zdroj: Scientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
Scientific Reports
ISSN: 2045-2322
DOI: 10.1038/s41598-019-56429-4
Popis: The aim of this study was to visualize the tumor propagation and surrounding mucosal field in radiography-based 3D model for advanced stage HNSCC and combine it with HPV genotyping and miRNA expression characterization of the visualized area. 25 patients with T1-3 clinical stage HNSCC were enrolled in mapping biopsy sampling. Biopsy samples were evaluated for HPV positivity and miR-21-5p, miR-143, miR-155, miR-221-5p expression in Digital Droplet PCR system. Significant miRNA expression differences of HPV positive tumor tissue biopsies were found for miR-21-5p, miR-143 and miR-221-5p compared to the HPV negative tumor biopsy series. Peritumoral mucosa showed patchy pattern alterations of miR-21-5p and miR-155 in HPV positive cases, while gradual change of miR-21-5p and miR-221-5p was seen in HPV negative tumors. In our study we found differences of the miRNA expression patterns among the HPV positive and negative tumorous tissues as well as the surrounding mucosal fields. The CT based 3D models of the cancer field and surrounding mucosal surface can be utilized to improve proper preoperative planning. Complex evaluation of HNSCC tissue organization field can elucidate the clinical and molecular differentiation of HPV positive and negative cases, and enhance effective organ saving therapeutic strategies.
Databáze: OpenAIRE
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