Phosphorus toxicity disrupts Rubisco activation and reactive oxygen species defence systems by phytic acid accumulation in leaves
Autor: | Naoki Hirotsu, Yuji Suzuki, Daisuke Takagi, Mao Suganami, Akihiro Ueda, Amane Makino, Ko Noguchi, Maki Kawai-Yamada, Atsuko Miyagi, Youshi Tazoe |
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Rok vydání: | 2020 |
Předmět: |
0106 biological sciences
0301 basic medicine Chloroplasts Phytic Acid Physiology Ribulose-Bisphosphate Carboxylase chemistry.chemical_element Plant Science Photosynthesis 01 natural sciences Superoxide dismutase Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound Ascorbate Peroxidases Cytosol Gene Expression Regulation Plant Plant Proteins chemistry.chemical_classification Reactive oxygen species Phytic acid biology Superoxide Dismutase Phosphorus RuBisCO food and beverages Oryza Enzyme Activation Plant Leaves 030104 developmental biology chemistry Biochemistry Toxicity biology.protein Reactive Oxygen Species 010606 plant biology & botany |
Zdroj: | Plant, cellenvironmentREFERENCE. 43(9) |
ISSN: | 1365-3040 |
Popis: | Phosphorus (P) is an essential mineral nutrient for plants. Nevertheless, excessive P accumulation in leaf mesophyll cells causes necrotic symptoms in land plants; this phenomenon is termed P toxicity. However, the detailed mechanisms underlying P toxicity in plants have not yet been elucidated. This study aimed to investigate the molecular mechanism of P toxicity in rice. We found that under excessive inorganic P (Pi) application, Rubisco activation decreased and photosynthesis was inhibited, leading to lipid peroxidation. Although the defence systems against reactive oxygen species accumulation were activated under excessive Pi application conditions, the Cu/Zn-type superoxide dismutase activities were inhibited. A metabolic analysis revealed that excessive Pi application led to an increase in the cytosolic sugar phosphate concentration and the activation of phytic acid synthesis. These conditions induced mRNA expression of genes that are activated under metal-deficient conditions, although metals did accumulate. These results suggest that P toxicity is triggered by the attenuation of both photosynthesis and metal availability within cells mediated by phytic acid accumulation. Here, we discuss the whole phenomenon of P toxicity, beginning from the accumulation of Pi within cells to death in land plants. |
Databáze: | OpenAIRE |
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