Early Postoperative Changes of HDL Subfraction Profile and HDL-Associated Enzymes After Laparoscopic Sleeve Gastrectomy

Autor: Tuna Bilecik, Mutay Aslan, Ramazan Eryilmaz, Cemal Özben Ensari, Serdar Dogan, Ibrahim Aslan
Rok vydání: 2013
Předmět:
Adult
Blood Glucose
Male
medicine.medical_specialty
Apolipoprotein B
Endocrinology
Diabetes and Metabolism
Enzyme-Linked Immunosorbent Assay
Body Mass Index
Phosphatidylcholine-Sterol O-Acyltransferase
chemistry.chemical_compound
High-density lipoprotein
Gastrectomy
Internal medicine
Weight Loss
Cholesterylester transfer protein
Humans
Insulin
Medicine
Electrophoresis
Gel
Two-Dimensional
Postoperative Period
chemistry.chemical_classification
Nutrition and Dietetics
Apolipoprotein A-I
biology
business.industry
Cholesterol
Cholesterol
HDL
Reverse cholesterol transport
nutritional and metabolic diseases
Cholesterol Ester Transfer Proteins
Obesity
Morbid
Treatment Outcome
Enzyme
Endocrinology
chemistry
biology.protein
Female
Laparoscopy
lipids (amino acids
peptides
and proteins)
Surgery
business
Body mass index
Abdominal surgery
Zdroj: ResearcherID
ISSN: 1708-0428
0960-8923
DOI: 10.1007/s11695-013-1011-2
Popis: This study aimed to determine early postoperative changes of LDL/HDL subfraction profile and HDL-associated enzymes following laparoscopic sleeve gastrectomy (LSG). Thirteen obese patients (mean body mass index (BMI) 52.74 ± 10.97 kg/m(2)) underwent LSG and normal weight control patients (mean BMI 23.56 ± 1.92 kg/m(2)) underwent laparoscopic abdominal surgery. Fasting blood samples were collected prior to surgery, at day 1 after surgery, and after postoperation oral feeding. LDL and HDL subfraction analysis was done by continuous disk polyacrylamide gel electrophoresis. Plasma levels of cholesteryl ester transfer protein (CETP), lecithin-cholesterol acyltransferase (LCAT), and apolipoprotein A-1 (apoA-I) were determined by enzyme-linked immunosorbent assay. Measurement of CETP and LCAT activity was performed via fluorometric analysis. LDL subfraction profile showed no change in both LSG and control group patients. No significant difference was observed in HDL cholesterol, HDL-subfraction distribution, and apoA-I levels in the control group. LSG patients showed a significant increase in HDL-large and a significant decrease in HDL-small fractions at postoperation day 1 compared to preoperation. HDL cholesterol significantly decreased and apoA-I significantly increased in LSG patients after postoperation oral feeding compared to both preoperation and postoperation day 1. Changes in HDL subfraction profile at postoperation day 1 after LSG were accompanied by a significant decrease in CETP protein, LCAT protein, and LCAT activity as compared to preoperation levels. Early changes in HDL subfraction profile and HDL-associated enzymes following LSG suggest that the surgical procedure, irrespective of changes in body weight, affects reverse cholesterol transport.
Databáze: OpenAIRE
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