The mitotic protein NuMA plays a spindle-independent role in nuclear formation and mechanics
| Autor: | John T. Canty, Ahmet Yildiz, Sophie Dumont, Ronja Houtekamer, Dorine Hintzen, Andrea Serra-Marques |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
1.1 Normal biological development and functioning
Cell Mitosis Cell Cycle Proteins Spindle Apparatus Biology Medical and Health Sciences Article Chromosomes 03 medical and health sciences 0302 clinical medicine Live cell imaging Genetics medicine Chromosomes Human Humans Fragmentation (cell biology) Interphase 030304 developmental biology Cell Nucleus 0303 health sciences Chemistry Chromosome Cell Biology DNA Biological Sciences Cell cycle Cell biology Cell nucleus HEK293 Cells medicine.anatomical_structure Mitotic exit Generic health relevance Nucleus 030217 neurology & neurosurgery Cell Cycle and Division Human Developmental Biology |
| Zdroj: | The Journal of cell biology, vol 219, iss 12 The Journal of Cell Biology |
| DOI: | 10.1101/2020.05.02.070680 |
| Popis: | NuMA disruption causes nuclear defects, and it was unclear if this is due to NuMA's essential spindle function. Serra-Marques et al. show that NuMA keeps chromosomes together at nuclear formation and promotes a mechanically robust nucleus independently of its spindle function. Eukaryotic cells typically form a single, round nucleus after mitosis, and failures to do so can compromise genomic integrity. How mammalian cells form such a nucleus remains incompletely understood. NuMA is a spindle protein whose disruption results in nuclear fragmentation. What role NuMA plays in nuclear integrity, and whether its perceived role stems from its spindle function, are unclear. Here, we use live imaging to demonstrate that NuMA plays a spindle-independent role in forming a single, round nucleus. NuMA keeps the decondensing chromosome mass compact at mitotic exit and promotes a mechanically robust nucleus. NuMA’s C terminus binds DNA in vitro and chromosomes in interphase, while its coiled-coil acts as a central regulatory and structural element: it prevents NuMA from binding chromosomes at mitosis, regulates its nuclear mobility, and is essential for nuclear formation. Thus, NuMA plays a structural role over the cell cycle, building and maintaining the spindle and nucleus, two of the cell’s largest structures. |
| Databáze: | OpenAIRE |
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