Role of microRNA modulation in the interferon-α/ribavirin suppression of HIV-1 in vivo
| Autor: | Andri Rauch, Xutao Deng, Joseph K. Wong, Mohamed Abdel-Mohsen, Evan S. Jacobs, Bruno Ledergerber, Teri Liegler, Satish K. Pillai, Huldrych F. Günthard, Philip J. Norris, Ali Danesh |
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| Přispěvatelé: | University of Zurich, Abdel-Mohsen, Mohamed |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Science
Immunology Viremia 610 Medicine & health HIV Infections 1100 General Agricultural and Biological Sciences Biology Interactome Microbiology 10234 Clinic for Infectious Diseases 1300 General Biochemistry Genetics and Molecular Biology In vivo Interferon Gene expression microRNA Ribavirin medicine Medicine and Health Sciences Humans Molecular Biology Regulation of gene expression 1000 Multidisciplinary Multidisciplinary Gene Expression Profiling Interferon-alpha Biology and Life Sciences Computational Biology virus diseases Cell Biology medicine.disease Hepatitis C 3. Good health Gene expression profiling MicroRNAs Infectious Diseases HIV-1 Medicine medicine.drug Research Article |
| Zdroj: | PLoS ONE, Vol 9, Iss 10, p e109220 (2014) Wong, Joseph; Abdel-Mohsen, M; Deng, X; Danesh, A; Liegler, T; Jacobs, ES; et al.(2014). Role of MicroRNA modulation in the interferon-α/ribavirin suppression of HIV-1 in vivo. UC San Francisco: Retrieved from: http://www.escholarship.org/uc/item/168311k8 PLoS ONE Abdel-Mohsen, Mohamed; Deng, Xutao; Danesh, Ali; Liegler, Teri; Jacobs, Evan S; Rauch, Andri; Ledergerber, Bruno; Norris, Philip J; Günthard, Huldrych F; Wong, Joseph K; Pillai, Satish K (2014). Role of MicroRNA Modulation in the Interferon-α/Ribavirin Suppression of HIV-1 In Vivo. PLoS ONE, 9(10), e109220. Public Library of Science 10.1371/journal.pone.0109220 |
| ISSN: | 1932-6203 |
| Popis: | BackgroundInterferon-α (IFN-α) treatment suppresses HIV-1 viremia and reduces the size of the HIV-1 latent reservoir. Therefore, investigation of the molecular and immunologic effects of IFN-α may provide insights that contribute to the development of novel prophylactic, therapeutic and curative strategies for HIV-1 infection. In this study, we hypothesized that microRNAs (miRNAs) contribute to the IFN-α-mediated suppression of HIV-1. To inform the development of novel miRNA-based antiretroviral strategies, we investigated the effects of exogenous IFN-α treatment on global miRNA expression profile, HIV-1 viremia, and potential regulatory networks between miRNAs and cell-intrinsic anti-HIV-1 host factors in vivo.MethodsGlobal miRNA expression was examined in longitudinal PBMC samples obtained from seven HIV/HCV-coinfected, antiretroviral therapy-naïve individuals before, during, and after pegylated interferon-α/ribavirin therapy (IFN-α/RBV). We implemented novel hybrid computational-empirical approaches to characterize regulatory networks between miRNAs and anti-HIV-1 host restriction factors.ResultsmiR-422a was the only miRNA significantly modulated by IFN-α/RBV in vivo (pConclusionsThe specific reduction of miR-422a is associated with exogenous IFN-α treatment, and likely contributes to the IFN-α suppression of HIV-1 through the enhancement of anti-HIV-1 restriction factor expression and regulation of genes involved in programmed cell death. Moreover, our regulatory network analysis presents additional candidate miRNAs that may be targeted to enhance anti-HIV-1 restriction factor expression in vivo. |
| Databáze: | OpenAIRE |
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