Berberine reduced blood pressure and improved vasodilation in diabetic rats
| Autor: | Zhijun Dai, Man-Jiang Xie, Yinbin Zhang, Bao-Feng Wang, Yu-Guang Ma, Zhongwei Wang, Liang Liang, Yun-Gang Bai |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Middle Cerebral Artery Time Factors Vascular smooth muscle Berberine Myocytes Smooth Muscle Cerebral arteries Gene Expression Blood Pressure Vasodilation 030204 cardiovascular system & hematology Pharmacology Muscle Smooth Vascular Diabetes Mellitus Experimental 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Diabetes mellitus medicine Animals Large-Conductance Calcium-Activated Potassium Channel alpha Subunits Molecular Biology Dose-Response Relationship Drug Vascular disease business.industry medicine.disease Streptozotocin Rats 030104 developmental biology Blood pressure chemistry Hyperglycemia business medicine.drug |
| Zdroj: | Journal of Molecular Endocrinology. 59:191-204 |
| ISSN: | 1479-6813 0952-5041 |
| DOI: | 10.1530/jme-17-0014 |
| Popis: | Hyperglycemia and hypertension are considered to be the two leading risk factors for vascular disease in diabetic patients. However, few pharmacologic agents could provide a combinational therapy for controlling hyperglycemia and hypertension at the same time in diabetes. The objectives of this study are to investigate whether berberine treatment could directly reduce blood pressure and identify the molecular mechanism underlying the vascular protection of berberine in diabetic rats. Berberine was intragastrically administered with different dosages of 50, 100 and 200 mg/kg/day to diabetic rats for 8 weeks since the injection of streptozotocin. The endothelium-dependent/-independent relaxation in middle cerebral arteries was investigated. The activity of large-conductance Ca2+-activated K+ channel (BKCa) was investigated by recording whole-cell currents, analyzing single-channel activities and assessing the expressions of α- and β1-subunit at protein or mRNA levels. Results of the study suggest that chronic administration of 100 mg/kg/day berberine not only lowered blood glucose but also reduced blood pressure and improved vasodilation in diabetic rats. Furthermore, berberine markedly increased the function and expression of BKCa β1-subunit in cerebral vascular smooth muscle cells (VSMCs) isolated from diabetic rats or when exposed to hyperglycemia condition. The present study provided initial evidences that berberine reduced blood pressure and improved vasodilation in diabetic rats by activation of BKCa channel in VSMCs, which suggested that berberine might provide a combinational therapy for controlling hyperglycemia and blood pressure in diabetes. Furthermore, our work indicated that activation of BKCa channel might be the underlying mechanism responsible for the vascular protection of berberine in diabetes. |
| Databáze: | OpenAIRE |
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