Safety studies of Nexrutine, bark extract of Phellodendron amurense through repeated oral exposure to rats for 28 days
| Autor: | Shamshad Alam, Payal Mandal, Pankaj Jagdale, Kausar M. Ansari, Anjaneya Ayanur |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Science (General) Pharmacology Safety evaluation Q1-390 chemistry.chemical_compound Internal medicine Medicine H1-99 Kidney Multidisciplinary Hematology Safety studies Triglyceride biology business.industry Cholesterol Single dose toxicity Repeated dose toxicity biology.organism_classification Social sciences (General) medicine.anatomical_structure chemistry visual_art Toxicity visual_art.visual_art_medium Phellodendron amurense Nexrutine Bark business Research Article |
| Zdroj: | Heliyon Heliyon, Vol 7, Iss 7, Pp e07654-(2021) |
| ISSN: | 2405-8440 |
| Popis: | Nexrutine (NX), a marketable herbal extract from a traditional Chinese herbal plant, Phellodendron amurense, is majorly used for the resolution of inflammation, gastroenteritis, and some tissue-specific cancer. Strategies for the identification of the safety of anticancer solutions of plant origin are an important area of study. The present investigation assesses the single and repeated dose (28 days) toxicity of NX following OECD guidelines 425 and 407, respectively. Briefly, to identify acute toxic properties of NX, a dose of 2000 mg/kg b. wt was administered once orally. Simultaneously, repeated dose toxicity was evaluated through daily administration of the three different doses (250, 500, 750 mg/kg b. wt) of NX for 28days. The single administration of NX showed no signs of toxicity and morbidity, suggesting LD50 of NX more than 2000 mg/kg b. wt. Furthermore, repeated dose exposure of NX for 28 days did not show any sign of toxicity. Hematology, serum biochemistry, and histopathological analysis also did not show any significant abnormalities. However, a marginal decrease in triglyceride, cholesterol, and glucose levels along with mild tubular degeneration in the kidney was also noticed in the high dose NX treatment group. Overall, the findings of the study suggest that NX is safe for use up to 500 mg/kg b.wt. Highlights • Single dose toxicity confirms LD50 of NX to be greater than 2000 mg/kg b. wt. • Repeated dose toxicity study used three doses of NX (250, 500, 750 mg/kg b. wt). • Minimal aberrations in hematology and biochemical parameters. • Histopathology depicts mild tubular degeneration at a high dose in the kidney. • No morbidity or mortality was recorded in both the experimental setups. Safety evaluation; Nexrutine; Single dose toxicity; Repeated dose toxicity. |
| Databáze: | OpenAIRE |
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