Arabidopsis thaliana cells: a model to evaluate the virulence of Pectobacterium carotovorum

Autor: Mohamed Kettani-Halabi, Raja Ait M'hand, Daniel Tran, Florence Val, Moulay Mustapha Ennaji, Patrice Meimoun, Meriam Terta, François Bouteau, Hayat El-Maarouf-Bouteau, K. Ibenyassine
Přispěvatelé: Laboratoire d'Electrophysiologie des Membranes (LEM) EA 3514, Université Paris Diderot - Paris 7 (UPD7), Laboratoire de virologie et Hygiène et Microbiologie (LVHM), Université Hassan II [Casablanca] (UH2MC)-Faculte des sciences et Techniques Mohammedia [Casablanca] (FSTM), Université Hassan II [Casablanca] (UH2MC)-Ministère de l'Enseignement supérieur,de la Recherche et de la Formation des cadres (Royaume du Maroc), UR5-UPM, Université Pierre et Marie Curie - Paris 6 (UPMC), Biologie des organismes et des populations appliquées à la protection des plantes (BIO3P), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, Université Hassan II [Casablanca] (UH2MC)-Ministere de l'Enseignement Superieur,de la recherche et de la formation des Cadres Royaume du Maroc, AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Recherche Agronomique (INRA), Faculte des sciences et Techniques Mohammedia [Casablanca] (FSTM), Université Hassan II [Casablanca] (UH2MC)-Université Hassan II [Casablanca] (UH2MC)-Ministere de l'Enseignement Superieur,de la recherche et de la formation des Cadres Royaume du Maroc, UMR BIO3p, Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST
Jazyk: angličtina
Rok vydání: 2010
Předmět:
0106 biological sciences
subsp carotovora
MESH: Cell Death
MESH: Hydrogen-Ion Concentration
MESH: Propiophenones
Physiology
chrysanthemi
MESH: Polysaccharide-Lyases
Arabidopsis
MESH: Anoxia
Pectobacterium carotovorum
MESH: Carbon Dioxide
01 natural sciences
Virulence factor
MESH: Dogs
MESH: Plant Diseases
Arabidopsis thaliana
MESH: Coronary Vessels
MESH: Animals
MESH: Arabidopsis
MESH: Heart Rate
MESH: Heart Septum
2. Zero hunger
0303 health sciences
biology
Cell Death
Effector
food and beverages
General Medicine
MESH: Blood Pressure
plant pathogen
Pectate lyase
MESH: Oxygen
atroseptica
Virulence Factors
Virulence
MESH: Coronary Circulation
potato diseases
Models
Biological
Microbiology
diversity
strains
03 medical and health sciences
erwinia-carotovora
Xanthomonas
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
030304 developmental biology
Plant Diseases
Polysaccharide-Lyases
MESH: Virulence Factors
fungi
MESH: Cardiac Output
MESH: Models
Biological
MESH: Pectobacterium carotovorum
biology.organism_classification
MESH: Vasodilator Agents
Quorum sensing
responses
identification
MESH: Heart Ventricles
Agronomy and Crop Science
010606 plant biology & botany
Zdroj: Molecular Plant-Microbe Interactions
Molecular Plant-Microbe Interactions, 2010, 23 (2), pp.139-43. ⟨10.1094/MPMI-23-2-0139⟩
Molecular Plant-Microbe Interactions, American Phytopathological Society, 2010, 23 (2), pp.139-43. ⟨10.1094/MPMI-23-2-0139⟩
ISSN: 0894-0282
DOI: 10.1094/MPMI-23-2-0139⟩
Popis: Pectobacterium carotovorum are economically important plant pathogens that cause plant soft rot. These enterobacteria display high diversity world-wide. Their pathogenesis depends on production and secretion of virulence factors such as plant cell wall–degrading enzymes, type III effectors, a necrosis-inducing protein, and a secreted virulence factor from Xanthomonas spp., which are tightly regulated by quorum sensing. Pectobacterium carotovorum also present pathogen-associated molecular patterns that could participate in their pathogenicity. In this study, by using suspension cells of Arabidopsis thaliana, we correlate plant cell death and pectate lyase activities during coinfection with different P. carotovorum strains. When comparing soft rot symptoms induced on potato slices with pectate lyase activities and plant cell death observed during coculture with Arabidopsis thaliana cells, the order of strain virulence was found to be the same. Therefore, Arabidopsis thaliana cells could be an alternative tool to evaluate rapidly and efficiently the virulence of different P. carotovorum strains.
Databáze: OpenAIRE
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