Macromolecular requirements for abrogation of Fv-1 restriction by murine leukemia viruses
| Autor: | Beryl M. Benjers, Robert H. Bassin, Judith G. Levin, G Duran-Troise, Brenda I. Gerwin, Alan Rein |
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| Jazyk: | angličtina |
| Rok vydání: | 1980 |
| Předmět: |
Hot Temperature
viruses Immunology RNA-dependent RNA polymerase Mice Inbred Strains Virus Replication Microbiology Virus Cell Line Viral Function Mice Capsid Viral entry Virology Murine leukemia virus Animals DNA synthesis biology Cytarabine RNA RNA-Directed DNA Polymerase biology.organism_classification Molecular biology Leukemia Virus Murine Viral replication Gene Expression Regulation Insect Science DNA Viral Dactinomycin RNA Viral Research Article |
| Popis: | The molecular basis of abrogation of Fv-1 restriction in mouse cells by murine leukemia virus was investigated. Two different lines of experimentation indicated that high-molecular-weight viral RNA is required for abrogation. First, the decay of abrogating ability of virus stocks heated at 43 degrees C was quantitatively correlated with a loss of intact virion 35S RNA. Second, Act D virions, which lack such RNA although they contain normal structural proteins, failed to abrogate. These findings imply that abrogation does not result from the mere entry of virion structural proteins into a cell. Additional data indicate that the role of viral RNA in abrogation is not that of a template for DNA synthesis. Virus particles lacking reverse transcriptase activity as a result of either mutation or heat inactivation exhibit abrogating activity even though they do not synthesize detectable viral DNA. In addition, abrogation was shown to take place in the presence of cytosine arabinoside, an inhibitor of DNA synthesis. Thus, abrogation does not depend on viral or cellular DNA synthesis, and the role of viral RNA in this process must involve some other function. The nature of this viral function and its occurrence in Fv-1 permissive cells are discussed. |
| Databáze: | OpenAIRE |
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