Metabolomic alternations of follicular fluid of obese women undergoing in-vitro fertilization treatment
| Autor: | Shan Xiang, Conghui Pang, Zhengao Sun, Jingyan Song, Jiayin Guo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Adult medicine.medical_specialty lcsh:Medicine Fertilization in Vitro Overweight Article Mass Spectrometry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Metabolomics Medical research Internal medicine Follicular phase medicine Humans Carnitine Obesity lcsh:Science 030219 obstetrics & reproductive medicine Multidisciplinary Fatty acid metabolism business.industry lcsh:R Lipid metabolism Lipid Metabolism Follicular fluid Follicular Fluid Metabolic pathway Oxidative Stress 030104 developmental biology Endocrinology chemistry lcsh:Q Female medicine.symptom business Systems biology Infertility Female Metabolic Networks and Pathways medicine.drug Chromatography Liquid |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-12 (2020) |
| ISSN: | 2045-2322 |
| Popis: | Obesity exerts negative effects on the metabolic homeostasis of cells in various tissues, but how it influences ovum metabolism is not fully understood. Previous studies demonstrate that oocyte genes that regulate oxidative stress, lipid metabolism, and inflammation are highly expressed in obese women. However, the metabolic effects of these genetic variations are not clear. To address this gap, we conducted an exploratory evaluation of follicular fluid (FF) metabolites in underweight, normal-weight, overweight, and obese women undergoing in vitro fertilization (IVF) treatment. The FF samples from the underweight (Group A, n = 40), normal-weight (Group B, n = 40), overweight (Group C, n = 40), and obese women (Group D, n = 40) were analyzed using ultra-performance liquid chromatography high-resolution mass spectrometry. A novel, high-coverage, semi-targeted metabolomics method (SWATH to MRM) and a targeted metabolomics method were employed to identify and verify the differential metabolites between the four groups. Sixteen differentially expressed FF metabolites were identified. Increase of BMI was associated with upregulation of 5 metabolites, ganoderiol H, LPI (18:3), sedoheptulose 1,7-bisphosphate, austalide L and 2 - {[hydroxyl (3-hydroxy-4-methoxyphenylmethylidene] amino} acetic acid, and downregulation of 5 metabolites, 1-phenyl-1,3-elcosanedione, retinol acetate, p-Cresol sulfate, setariol and arachidonyl carnitine. These metabolites were enriched in different metabolic pathways of retinol metabolism and fatty acid metabolism. These obesity-related differential metabolites provide a pathogenesis mechanism that explains the decline of oocyte development during obesity. These results suggest that obesity affects follicular environment prior to pregnancy, a time-window that may be important for lifestyle interventions to decrease obesity levels. |
| Databáze: | OpenAIRE |
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