Effect of the natural compound trans‑resveratrol on human MCM4 gene transcription
| Autor: | Marie Tanaka, Monami Kusaka, Chihiro Katsuda, Masashi Asai, Motohiro Akui, Fumiaki Uchiumi, Sei-ichi Tanuma |
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| Rok vydání: | 2020 |
| Předmět: |
Cancer Research
SPI1 Expression vector Transcription Genetic Chemistry HL-60 Cells Promoter General Medicine Transfection Molecular biology Minichromosome Maintenance Complex Component 4 Oncology Resveratrol Transcription (biology) Cell Line Tumor Proto-Oncogene Proteins Gene expression Trans-Activators Humans Electrophoretic mobility shift assay Promoter Regions Genetic Gene Transcription factor |
| Zdroj: | Oncology Reports. 44:283-292 |
| ISSN: | 1791-2431 1021-335X |
| DOI: | 10.3892/or.2020.7598 |
| Popis: | trans‑Resveratrol (Rsv) is a natural compound contained in red wine and grape skins that has various beneficial effects for organisms such as lengthening of their life span. Rsv induces expression of the human TP53 and HELB genes, which are involved in the regulation of DNA maintenance. In the present study, a luciferase expression vector containing 309 bp of the 5' upstream end of the human MCM4 gene was transfected into HeLa S3 cells. A luciferase assay revealed that Rsv treatment increased the minichromosome maintenance 4 (MCM4) gene promoter activity by GC‑box and GGAA (TTCC) motifs. Electro phoretic mobility shift assay revealed that the specific binding factor (complex) contains PU.1 (SPI1). Quantitative reverse transcription‑polymerase chain reaction analysis indicated that MCM4 gene expression was transiently induced by Rsv. Moreover, western blotting revealed that the SP1/PU.1 ratio markedly increased after Rsv treatment, indicating that a balance or profile of these transcription factors may control Rsv‑inducible initiation of transcription. These observations indicated that the beneficial effects of Rsv can be attributed to induction of the chromosomal DNA maintenance factor encoding gene expression. |
| Databáze: | OpenAIRE |
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