Antiangiogenesis, Loss of Cell Adhesion and Apoptosis Are Involved in the Antitumoral Activity of Proteases from V. cundinamarcensis (C. candamarcensis) in Murine Melanoma B16F1

Autor: Cinthia Figueiredo, Carlos E. Salas, Celso Tarso Rodrigues Viana, Dalton Dittz, Ricardo Toshio Fujiwara, Miriam T. P. Lopes, Fernanda O. Lemos, Elaine M. Souza-Fagundes, Silvia Passos Andrade
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Male
Skin Neoplasms
Angiogenesis
Melanoma
Experimental
Angiogenesis Inhibitors
lcsh:Chemistry
Extracellular matrix
chemistry.chemical_compound
Mice
Transforming Growth Factor beta
lcsh:QH301-705.5
Spectroscopy
Plant Proteins
biology
Carica
Proteolytic enzymes
apoptosis
General Medicine
melanoma B16F1
Computer Science Applications
Vascular endothelial growth factor
medicine.anatomical_structure
Treatment Outcome
Biochemistry
antitumoral
V. cundinamarcensis
Catalysis
Article
Inorganic Chemistry
Cell Line
Tumor
medicine
Cell Adhesion
Animals
Physical and Theoretical Chemistry
Cell adhesion
Fibroblast
Molecular Biology
Tumor Necrosis Factor-alpha
Organic Chemistry
Transforming growth factor beta
Antineoplastic Agents
Phytogenic
lcsh:Biology (General)
lcsh:QD1-999
chemistry
biology.protein
Cancer research
proteases
anchorage loss
Wound healing
Peptide Hydrolases
Zdroj: International Journal of Molecular Sciences
Volume 16
Issue 4
Pages 7027-7044
International Journal of Molecular Sciences, Vol 16, Iss 4, Pp 7027-7044 (2015)
ISSN: 1422-0067
DOI: 10.3390/ijms16047027
Popis: The proteolytic enzymes from V. cundinamarcensis latex, (P1G10), display healing activity in animal models following various types of lesions. P1G10 or the purified isoforms act as mitogens on fibroblast and epithelial cells by stimulating angiogenesis and wound healing in gastric and cutaneous ulcers models. Based on evidence that plant proteinases act as antitumorals, we verified this effect on a murine melanoma model. The antitumoral effect analyzed mice survival and tumor development after subcutaneous administration of P1G10 into C57BL/6J mice bearing B16F1 low metastatic melanoma. Possible factors involved in the antitumoral action were assessed, i.e., cytotoxicity, cell adhesion and apoptosis in vitro, haemoglobin (Hb), vascular endothelial growth factor (VEGF), tumor growth factor-β (TGF-β), tumor necrosis factor-α (TNF-α) content and N-acetyl-glucosaminidase (NAG) activity. We observed that P1G10 inhibited angiogenesis measured by the decline of Hb and VEGF within the tumor, and TGF-β displayed a non-significant increase and TNF-α showed a minor non-significant reduction. On the other hand, there was an increase in NAG activity. In treated B16F1 cells, apoptosis was induced along with decreased cell binding to extracellular matrix components (ECM) and anchorage, without impairing viability.
Databáze: OpenAIRE
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