Antiangiogenesis, Loss of Cell Adhesion and Apoptosis Are Involved in the Antitumoral Activity of Proteases from V. cundinamarcensis (C. candamarcensis) in Murine Melanoma B16F1
Autor: | Cinthia Figueiredo, Carlos E. Salas, Celso Tarso Rodrigues Viana, Dalton Dittz, Ricardo Toshio Fujiwara, Miriam T. P. Lopes, Fernanda O. Lemos, Elaine M. Souza-Fagundes, Silvia Passos Andrade |
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Jazyk: | angličtina |
Rok vydání: | 2015 |
Předmět: |
Male
Skin Neoplasms Angiogenesis Melanoma Experimental Angiogenesis Inhibitors lcsh:Chemistry Extracellular matrix chemistry.chemical_compound Mice Transforming Growth Factor beta lcsh:QH301-705.5 Spectroscopy Plant Proteins biology Carica Proteolytic enzymes apoptosis General Medicine melanoma B16F1 Computer Science Applications Vascular endothelial growth factor medicine.anatomical_structure Treatment Outcome Biochemistry antitumoral V. cundinamarcensis Catalysis Article Inorganic Chemistry Cell Line Tumor medicine Cell Adhesion Animals Physical and Theoretical Chemistry Cell adhesion Fibroblast Molecular Biology Tumor Necrosis Factor-alpha Organic Chemistry Transforming growth factor beta Antineoplastic Agents Phytogenic lcsh:Biology (General) lcsh:QD1-999 chemistry biology.protein Cancer research proteases anchorage loss Wound healing Peptide Hydrolases |
Zdroj: | International Journal of Molecular Sciences Volume 16 Issue 4 Pages 7027-7044 International Journal of Molecular Sciences, Vol 16, Iss 4, Pp 7027-7044 (2015) |
ISSN: | 1422-0067 |
DOI: | 10.3390/ijms16047027 |
Popis: | The proteolytic enzymes from V. cundinamarcensis latex, (P1G10), display healing activity in animal models following various types of lesions. P1G10 or the purified isoforms act as mitogens on fibroblast and epithelial cells by stimulating angiogenesis and wound healing in gastric and cutaneous ulcers models. Based on evidence that plant proteinases act as antitumorals, we verified this effect on a murine melanoma model. The antitumoral effect analyzed mice survival and tumor development after subcutaneous administration of P1G10 into C57BL/6J mice bearing B16F1 low metastatic melanoma. Possible factors involved in the antitumoral action were assessed, i.e., cytotoxicity, cell adhesion and apoptosis in vitro, haemoglobin (Hb), vascular endothelial growth factor (VEGF), tumor growth factor-β (TGF-β), tumor necrosis factor-α (TNF-α) content and N-acetyl-glucosaminidase (NAG) activity. We observed that P1G10 inhibited angiogenesis measured by the decline of Hb and VEGF within the tumor, and TGF-β displayed a non-significant increase and TNF-α showed a minor non-significant reduction. On the other hand, there was an increase in NAG activity. In treated B16F1 cells, apoptosis was induced along with decreased cell binding to extracellular matrix components (ECM) and anchorage, without impairing viability. |
Databáze: | OpenAIRE |
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