Antibacterial potential of Forsythia suspensa polysaccharide against resistant Enterobacter cloacae with SHV‐12 extended‐spectrum β‐lactamase (ESBL)
| Autor: | Zhen Guo, Yi Zhao, Zhen Jia, Zhujin Xu, Zuguo Zhao, Jing Chen, Jun Liu, Liyao Lin |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
food.ingredient Enterobacter cloacae producing SHV‐12 ESBL Forsythia suspense polysaccharide Microbial Sensitivity Tests Bacterial growth Chemical Fractionation beta-Lactam Resistance beta-Lactamases Microbiology 03 medical and health sciences 0302 clinical medicine Forsythia food antibacterial activity Polysaccharides Enterobacter cloacae polycyclic compounds Agar Pathogen Forsythia suspensa biology Chemistry Plant Extracts Biofilm Cell Biology Original Articles Gene Expression Regulation Bacterial biochemical phenomena metabolism and nutrition biology.organism_classification bacterial infections and mycoses Anti-Bacterial Agents 030104 developmental biology 030220 oncology & carcinogenesis Biofilms Molecular Medicine bacteria Original Article Antibacterial activity |
| Zdroj: | Journal of Cellular and Molecular Medicine |
| ISSN: | 1582-4934 1582-1838 |
| Popis: | In this study, a homogenous polysaccharide (FSP), with an average molecular weight of 9.08 × 104 Da, was isolated from Forsythia suspense and its antibacterial potential against Enterobacter cloacae producing SHV‐12 ESBL was investigated. Growth kinetics, in vitro competition and biofilm formation experiments demonstrated that SHV‐12 ESBL contributed to a fitness benefit to E cloacae strain. The antibacterial activity of FSP (2.5, 5.0 and 10.0 μg/mL) was tested against E cloacae bearing SHV‐12 ESBL gene using bacterial sensitivity, agar bioassay and agar well diffusion assays. It was found that the addition of FSP demonstrated potent antibacterial activities against this bacterial as showed by the decrease of bacterial growth and the increase of the inhibition zone diameter. Furthermore, SHV‐12 ESBL gene expression was decreased in E cloacae strain following different FSP treatment in a concentration‐dependent manner. In conclusion, these data showed that FSP exhibited potent good antibacterial activity against E cloacae producing SHV‐12 ESBL via inhibition of SHV‐12 ESBL gene expression, which may promote the development of novel natural antibacterial agents to treat infections caused by this drug‐resistant bacterial pathogen. |
| Databáze: | OpenAIRE |
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