Antihypertensive Effects of Virgin Olive Oil (Unfiltered) Low Molecular Weight Peptides with ACE Inhibitory Activity in Spontaneously Hypertensive Rats
| Autor: | Juan Duarte, Juan María Alcaide-Hidalgo, Miguel Romero, Eduardo López-Huertas |
|---|---|
| Přispěvatelé: | European Commission, Ministerio de Economía y Competitividad (España), Consejo Superior de Investigaciones Científicas (España) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male hypertension Peptide lcsh:TX341-641 Angiotensin-Converting Enzyme Inhibitors Blood Pressure Tandem mass spectrometry Article 03 medical and health sciences Rats Inbred SHR medicine Animals IC50 Olive Oil Antihypertensive Agents ACE chemistry.chemical_classification 030109 nutrition & dietetics Nutrition and Dietetics Fast protein liquid chromatography unfiltered virgin olive oil In vitro Amino acid Molecular Weight 030104 developmental biology Enzyme chemistry Biochemistry ACE inhibitor peptides spontaneously hypertensive rats lcsh:Nutrition. Foods and food supply Food Science medicine.drug |
| Zdroj: | Nutrients Volume 12 Issue 1 Nutrients, Vol 12, Iss 1, p 271 (2020) Digibug. Repositorio Institucional de la Universidad de Granada instname Digital.CSIC. Repositorio Institucional del CSIC |
| ISSN: | 2072-6643 |
| DOI: | 10.3390/nu12010271 |
| Popis: | The low molecular weight peptide composition of virgin olive oil (VOO) is mostly unknown. We hypothesised that unfiltered VOO could possess low molecular weight peptides with antihypertensive activity. We produced unfiltered VOO and obtained a water-soluble peptide extract from it. The peptides were separated by size-exclusion using fast protein liquid chromatography, and the low molecular weight fraction was analysed by nanoscale liquid chromatography-Orbitrap coupled with tandem mass spectrometry and de novo sequencing. We selected 23 peptide sequences containing between 6 and 9 amino acids and molecular masses ranging 698&ndash 1017 Da. Those peptides were chemically synthesised and their angiotensin-converting enzyme (ACE) inhibitory activity was studied in vitro. Seven peptides showed a strong activity, with half maximal inhibitory concentration (IC50) < 10 µ m. The antihypertensive effects of the four most active synthesised ACE inhibitor peptides were studied in spontaneously hypertensive rats (SHR). Acute oral administration of synthetic peptides RDGGYCC and CCGNAVPQ showed antihypertensive activity in SHR. We conclude that unfiltered VOO naturally contains low molecular weight peptides with specific ACE inhibitory activity and antihypertensive effects in SHR. |
| Databáze: | OpenAIRE |
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