Immobilization of sodium alginate sulfates on polysulfone ultrafiltration membranes for selective adsorption of low-density lipoprotein
| Autor: | Jian-Da Cao, Xiao-Jun Huang, Wen Wu, Ping Lan, Wei Wang |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Alginates
Polymers Surface Properties Sodium Biomedical Engineering Ultrafiltration chemistry.chemical_element Enzyme-Linked Immunosorbent Assay Biochemistry Biomaterials chemistry.chemical_compound Glucuronic Acid Spectroscopy Fourier Transform Infrared Sulfones Polysulfone Amines Molecular Biology Acrylamide Chromatography Sulfates Hexuronic Acids Membranes Artificial General Medicine Lipoproteins LDL Membrane chemistry LDL apheresis Selective adsorption Chemical binding Adsorption Glutaraldehyde Biotechnology |
| Zdroj: | Acta Biomaterialia. 10:234-243 |
| ISSN: | 1742-7061 |
| DOI: | 10.1016/j.actbio.2013.08.032 |
| Popis: | A novel method for the immobilization of sodium alginate sulfates (SAS) on polysulfone (PSu) ultrafiltration membranes to achieve selective adsorption of low-density lipoprotein (LDL) was developed, which involved the photoinduced graft polymerization of acrylamide on the membrane and the Hofmann rearrangement reaction of grafted acrylamide followed by chemical binding of SAS with glutaraldehyde. The surface modification processes were confirmed by attenuated total reflectance Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy characterization. Zeta potential and water contact angle measurements were performed to investigate the surface charge and wettability of the membranes. An enzyme-linked immunosorbent assay was used to measure the binding of LDL on plain and modified PSu membranes. It was found that the PSu membrane immobilized with sodium alginate sulfates (PSu-SAS) greatly enhanced the selective adsorption of LDL from protein solutions and the absorbed LDL could be easily eluted with sodium chloride solution, indicating a specific and reversible binding of LDL to SAS, mainly driven by electrostatic forces. Furthermore, the PSu-SAS membrane showed good blood compatibility as examined by platelet adhesion. The results suggest that the PSu-SAS membranes are promising for application in simultaneous hemodialysis and LDL apheresis therapy. |
| Databáze: | OpenAIRE |
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