A biophysical approach to quantify skeletal stem cells trans-differentiation as a model for the study of osteoporosis

Autor: Francesca Sbrana, Paola Gavazzo, Massimo Vassalli, Loredana Petecchia, Federica Viti
Rok vydání: 2017
Předmět:
0301 basic medicine
Cellular differentiation
Holography
Biochemistry
0302 clinical medicine
Quantitative phase imaging
Adipocytes
Microscopy
Phase-Contrast
Cells
Cultured
education.field_of_study
Adipocyte
Osteoblast
Cell Differentiation
Cell biology
medicine.anatomical_structure
Adipogenesis
030220 oncology & carcinogenesis
Real time qPCR
Stem cell
Fatty Acid-Binding Protein 7
medicine.medical_specialty
Population
Osteocalcin
Biophysics
Bone Marrow Cells
Biology
Real-Time Polymerase Chain Reaction
Models
Biological
Biophysical Phenomena
03 medical and health sciences
Imaging
Three-Dimensional
Internal medicine
medicine
Humans
Cell Lineage
education
Osteoblasts
Mechanism (biology)
Morphometry
Tumor Suppressor Proteins
Organic Chemistry
Mesenchymal stem cell
Mesenchymal Stem Cells
030104 developmental biology
Endocrinology
Cell Transdifferentiation
Osteoporosis
RNA
Bone marrow
Zdroj: Biophysical chemistry
229 (2017): 84–92. doi:10.1016/j.bpc.2017.05.011
info:cnr-pdr/source/autori:Petecchia L, Viti F, Sbrana F, Vassalli M, Gavazzo P/titolo:A biophysical approach to quantify skeletal stem cells trans-differentiation as a model for the study of osteoporosis/doi:10.1016%2Fj.bpc.2017.05.011/rivista:Biophysical chemistry (Print)/anno:2017/pagina_da:84/pagina_a:92/intervallo_pagine:84–92/volume:229
ISSN: 1873-4200
Popis: The stroma of human bone marrow contains a population of skeletal stem cells (hBM-MSC) which are common ancestors, among the others, of osteoblasts and adipocytes. It has been proposed that the imbalance between hBM-MSC osteogenesis and adipogenesis, which naturally accompanies bone marrow senescence, may contribute to the development of bone-associated diseases, like osteoporosis. The possibility to reproduce this mechanism in vitro has been demonstrated, providing a good model to disclose the details of the complex bone-fat generation homeostasis. Nevertheless, the lack of a simple approach to quantitatively assess the actual stage of a cellular population hindered the adoption of this in vitro model. In this work, the direct differentiation of hBM-MSCs towards a single (osteo or adipo) lineage was characterized using quantitative biophysical and biological approaches, together with the parallel process of trans-differentiation from one lineage to the other. The results confirm that the original plasticity of hBM-MSCs is maintained along the initial stages of the differentiation, showing that in vitro conversion of pre-osteoblasts into adipocytes and, vice versa, of pre-adipocytes into osteoblasts is extremely efficient, comparable with the direct differentiation. Moreover, a method based on digital holography is proposed, providing a quantitative indication of the phenotype stage along differentiation.
Databáze: OpenAIRE
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