Engineering microenvironment for expansion of sensitive anchorage-dependent mammalian cells
| Autor: | Mah-Lee Ng, Chao Yin, Pao-Chun Lin, Cho-Hong Goh, C. H. Quek, Hanry Yu, Hai-Quan Mao, Kam W. Leong, Ser-Mien Chia, Xi Xu |
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| Rok vydání: | 2005 |
| Předmět: |
Drug Compounding
Cell Cell Culture Techniques Bioengineering Nanotechnology Biology PC12 Cells Applied Microbiology and Biotechnology Buffer (optical fiber) Tissue engineering In vivo medicine Animals Humans Cell Proliferation chemistry.chemical_classification Cell growth General Medicine Polymer Rats medicine.anatomical_structure chemistry Biophysics Collagen Stem cell Ex vivo Biotechnology |
| Zdroj: | Journal of Biotechnology. 118:434-447 |
| ISSN: | 0168-1656 |
| Popis: | Tissue engineering involves ex vivo seeding of anchorage-dependent mammalian cells onto scaffolds, or transplanting cells in vivo. The cell expansion currently requires repeated cell detachment from solid substrata by enzymatic, chemical or mechanical means. The report here presents a high yield three-dimensional culture and harvest system circumventing the conventional detachment requirements. Cells mixed with dilute cationic collagen were microencapsulated within an ultra-thin shell of synthetic polymers. The cationic collagen could rapidly form a conformal layer of collagen fibers around cells to support cell proliferation and functions. The collagen could be readily removed from cells with a buffer rinse after harvesting from the fragile microcapsules. The cells harvested from this system demonstrate improved attachment, morphology and functions over conventionally cultured cells, upon binding to ligand-conjugated polymer surfaces. The harvested cells can be re-encapsulated and allowed to proliferate again, or used immediately in applications. |
| Databáze: | OpenAIRE |
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