Lactoferrin Ameliorates Dry Eye Disease Potentially through Enhancement of Short-Chain Fatty Acid Production by Gut Microbiota in Mice

Autor: Motoko Kawashima, Kazuo Tsubota, Toshihiro Imada, Shinji Fukuda, Shigeru Nakamura, Samuel Connell, Hiromitsu Yamamoto
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Administration
Oral
Gut flora
Systemic inflammation
Mice
dry eye
fluids and secretions
Tear secretion
Biology (General)
Spectroscopy
chemistry.chemical_classification
biology
Lactoferrin
Chemistry
Short-chain fatty acid
General Medicine
Anti-Bacterial Agents
Computer Science Applications
lactoferrin
Treatment Outcome
Cytokines
Dry Eye Syndromes
Female
medicine.symptom
Signal Transduction
DNA
Bacterial
QH301-705.5
Protective Agents
Article
Catalysis
Proinflammatory cytokine
Microbiology
Inorganic Chemistry
Immune system
Vancomycin
medicine
Animals
Physical and Theoretical Chemistry
Molecular Biology
QD1-999
Inflammation
gut microbiota
Organic Chemistry
Fatty acid
Fatty Acids
Volatile
biology.organism_classification
Gastrointestinal Microbiome
lacrimal gland
Mice
Inbred C57BL
Disease Models
Animal
stomatognathic diseases
Tears
biology.protein
short-chain fatty acid
Zdroj: International Journal of Molecular Sciences, Vol 22, Iss 12384, p 12384 (2021)
International Journal of Molecular Sciences
Volume 22
Issue 22
ISSN: 1661-6596
1422-0067
Popis: Lactoferrin is a glycoprotein found at high concentrations within exocrine secretions, including tears. Low levels of lactoferrin have been implicated in the loss of tear secretion and ageing. Furthermore, lactoferrin possesses a range of functionalities, including anti-inflammatory properties and the ability to modulate the gut microbiota. Expanding evidence demonstrates a crucial role of the gut microbiota in immune regulation and development. The specific composition of bacterial species of the gut has a profound influence on local and systemic inflammation, leading to a protective capacity against a number of inflammatory diseases, potentially by the induction of regulatory immune cells. In this study, we demonstrated that oral administration of lactoferrin maintains tear secretion in a restraint and desiccating stress induced mouse model of dry eye disease. Furthermore, we revealed that lactoferrin induces the reduction of inflammatory cytokines, modulates gut microbiota, and induces short-chain fatty acid production. Whereas, the antibiotic vancomycin abrogates the effects of lactoferrin on dry eye disease and significantly reduces short-chain fatty acid concentrations. Therefore, this protective effect of LF against a mice model of DED may be explained by our observations of an altered gut microbiota and an enhanced production of immunomodulatory short-chain fatty acids.
Databáze: OpenAIRE
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