RNA-dependent genome processing during nuclear differentiation: the model systems of stichotrichous ciliates
| Autor: | Hans J. Lipps, Mariusz Nowacki, Gloria Fuhrmann, Estienne C. Swart |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Cancer Research
Gene Dosage Computational biology Biology Oxytricha Models Biological Gene dosage DNA sequencing Epigenesis Genetic Genomic Imprinting 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Macronucleus Gene duplication Genetics RNA Small Nucleolar Ciliophora Gene 030304 developmental biology Cell Nucleus 0303 health sciences Gene Amplification RNA biology.organism_classification chemistry 030220 oncology & carcinogenesis DNA |
| Zdroj: | Epigenomics |
| ISSN: | 1750-192X 1750-1911 |
| Popis: | We introduce ciliated protozoa, and more specifically the stichotrichous ciliates Oxytricha and Stylonychia, as biological model systems for the analysis of programmed DNA-reorganization processes during nuclear differentiation. These include DNA excision, DNA elimination, reordering of gene segments and specific gene amplification. We show that small nuclear RNAs specify DNA sequences to be excised or retained, but also discuss the need for a RNA template molecule derived from the parental nucleus for these processes. This RNA template guides reordering of gene segments to become functional genes and determines gene copy number in the differentiated nucleus. Since the template is derived from the parental macronucleus, gene reordering and DNA amplification are inherited in a non-Mendelian epigenetic manner. |
| Databáze: | OpenAIRE |
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