Phospholipase Cγ2 Provides Survival Signals via Bcl2 and A1 in Different Subpopulations of B Cells
| Autor: | Demin Wang, Renren Wen, Stephan W. Morris, Yuhong Chen, Shoua Yang, James Schuman, Liquan Xue |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Time Factors
Cell Survival Blotting Western B-cell receptor Naive B cell Apoptosis Cell Separation Phospholipase Biology Biochemistry Flow cytometry Mice In Situ Nick-End Labeling medicine Animals Humans Replication Protein C Molecular Biology Cells Cultured B cell Bone Marrow Transplantation B-Lymphocytes medicine.diagnostic_test Phospholipase C gamma Reverse Transcriptase Polymerase Chain Reaction breakpoint cluster region Cell Biology Flow Cytometry Molecular biology Rats Cell biology DNA-Binding Proteins Retroviridae medicine.anatomical_structure Proto-Oncogene Proteins c-bcl-2 Type C Phospholipases Signal transduction Spleen Signal Transduction |
| Zdroj: | Journal of Biological Chemistry. 278:43654-43662 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m307318200 |
| Popis: | PLCgamma2 plays a critical role in B cell receptor (BCR) signaling and its targeted deletion results in defective B cell development and function. Here, we show that PLCgamma2 deficiency specifically blocks B cell maturation at the transitional type 2 (T2) to follicular (FO) B cell transition and the PLCgamma2 pathway regulates survival of B cells. BCR-induced apoptosis is dramatically enhanced in all subsets of splenic PLCgamma2-deficient B cells, especially in T2 and FO B cell subpopulations. We also find that all splenic PLCgamma2-deficient B cell subpopulations express abnormally low levels of Bcl-2 protein. In addition, PLCgamma2 deficiency disrupts BCR-mediated induction of A1 expression. Enforced expression of Bcl-2 prevents BCR-induced apoptosis in all splenic PLCgamma2-deficient B cell subpopulations and partially restores the numbers of PLCgamma2-deficient FO B cells. In contrast to Bcl-2, enforced expression of A1 preferentially prevents BCR-induced apoptosis in PLCgamma2-deficient FO B cells and partially restores the numbers of these B cells. Therefore, the PLCgamma2 pathway provides a survival signal via regulation of Bcl-2 in all splenic B cell subpopulations and via additional induction of A1 in mature FO B cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|