Physical exercise stimulates salivary secretion of cystatins
| Autor: | Leandro T. Oliveira, Martha M. Sorenson, D. William Provance, Diego Viana Gomes, Marcelo de Lima Sant’Anna, Sergio Tadeu Farinha Marques, Verônica P. Salerno |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Saliva Physiology Social Sciences urologic and male genital diseases Salivary Glands Running 0302 clinical medicine Acinus Medicine and Health Sciences Psychology Public and Occupational Health Cells Cultured reproductive and urinary physiology Multidisciplinary biology Chemistry Drugs Adrenergic beta-Agonists Submandibular Glands Submandibular gland Sports Science female genital diseases and pregnancy complications Body Fluids medicine.anatomical_structure Salivary Cystatins Medicine Cystatin Anatomy Research Article Sports Adult Agonist medicine.medical_specialty medicine.drug_class Science Adrenergic beta-Antagonists Submandibular Gland 03 medical and health sciences Exocrine Glands stomatognathic system Internal medicine medicine Humans Secretion Sports and Exercise Medicine Exercise Pharmacology Behavior Biological Locomotion Isoproterenol Biology and Life Sciences Physical Activity 030229 sport sciences 030104 developmental biology Endocrinology Cystatin C Physical Fitness biology.protein Recreation Physiological Processes Digestive System |
| Zdroj: | PLoS ONE, Vol 14, Iss 10, p e0224147 (2019) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Physical exercise is known to activate the sympathetic nervous system, which influences the production of saliva from salivary glands. Our examination of saliva collected from highly trained athletes before and after a number of physical competititions showed an increase in the secretion of S-type cystatins and cystatin C as a subacute response to aerobic and anaerobic exercise. The elevation in salivary cystatins was transient and the recovery time course differed from that of amylase and other salivary proteins. An in vitro assay was developed based on a cell line from a human submandibular gland (HSG) that differentiated into acinus-like structures. Treatments with the β-adrenergic agonist isoproterenol caused a shift in the intracellular distribution of S-type cystatins and cystatin C, promoting their accumulation at the outer regions of the acinus prior to release and suggesting the activation of a directional transport involving co-migration of both molecules. In another treatment using non-differentiated HSG cells, it was evident that both expression and secretion of cystatin C increased upon addition of the β-adrenergic agonist, and these effects were essentially eliminated by the antagonist propranolol. The HSG cell line appears to have potential as a model for exploring the mechanism of cystatin secretion, particularly the S-type cystatins that originate primarily in the submandibular glands. |
| Databáze: | OpenAIRE |
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