Fast measurement of phosphates and ammonium in fermentation-like media:A feasibility study
| Autor: | Nils Arneborg, Anna Eliasson Lantz, Thomas Skov, Marta Bevilacqua, Katrin Pontius, Flemming H. Larsen, Giulia Praticò |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Analyte Infrared spectroscopy Bioengineering 01 natural sciences Phosphates Chemometrics 03 medical and health sciences chemistry.chemical_compound 010608 biotechnology Ammonium Compounds Partial least squares regression Ammonium Least-Squares Analysis Spectroscopy Molecular Biology Mid-Infrared spectroscopy Near-Infrared spectroscopy 030304 developmental biology 0303 health sciences Spectroscopy Near-Infrared Near-infrared spectroscopy General Medicine chemistry Calibration Fermentation Process monitoring Feasibility Studies Environmental science Biological system Biotechnology |
| Zdroj: | Pontius, K, Praticò, G, Larsen, F H, Skov, T, Arneborg, N, Lantz, A E & Bevilacqua, M 2020, ' Fast measurement of phosphates and ammonium in fermentation-like media : A feasibility study ', New Biotechnology, vol. 56, pp. 54-62 . https://doi.org/10.1016/j.nbt.2019.11.006 Pontius, K, Praticò, G, Larsen, F H, Skov, T, Arneborg, N, Eliasson Lantz, A & Bevilacqua, M 2020, ' Fast measurement of phosphates and ammonium in fermentation-like media: a feasibility study ', New Biotechnology, vol. 56, pp. 54-62 . https://doi.org/10.1016/j.nbt.2019.11.006 |
| DOI: | 10.1016/j.nbt.2019.11.006 |
| Popis: | Real-time monitoring of bioprocesses plays a key-role in modern industries, providing new information on full-scale production, thus enabling control of the process and allowing it to run at optimal conditions while minimizing waste. Monitoring of phosphates and ammonium in fermentation processes has a twofold interest: they are important nutrients for living organisms while at the same time constituting environmental nutrient pollutants, for which unnecessary use and disposal must be avoided. In this report, the possibility of simultaneous analysis of phosphates and ammonium in fermentations was verified using spectroscopy-based methods combined with chemometrics to construct calibration models. To achieve this, the models were based on synthetic samples mimicking real fermentation media, providing a dataset where the analytes were completely uncorrelated. Different at-line techniques (mid- and near- infrared spectroscopy, MIR and NIR) were evaluated for their ability to monitor quickly both analytes, in a wide range of concentrations (10−100 mM), in three media of different complexities. Partial Least Squares (PLS) models on MIR spectroscopy gave very good results, with prediction errors lower than 5 % for both analytes in all datasets. In contrast, the results for PLS models on NIR spectroscopy were inferior (prediction errors between 3 and 26 %) for both analytes, as, in the case of phosphate, it could be demonstrated that the model was based on based on indirect predictions. |
| Databáze: | OpenAIRE |
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